Asha Lakkavaram
Grovedale,Australia
Work Preference
Summary
A results-driven professional with comprehensive background in diverse roles from biology researcher, clinical researcher, AI generalist to teaching aide. Known for enhancing operational workflows and contributing to cross-functional team success. Reliable, adaptable, and skilled in multitasking and conflict resolution.
Overview
24
24
years of professional experience
Work History
Lab technician
Deakin University
Waurn Ponds, Victoria, Australia
05.2019 - 10.2019
- Performing cell culture, growing and freezing panels of cancer cell lines
- Preparation of cell extracts for detection of specific antigens by western blots
- Creating SOPs, data analysis, preparing reagents and maintaining inventory.
Lab technician
Deakin University
Waurn Ponds, Victoria, Australia
01.2017 - 10.2019
- Investigating pathology of malaria in severe malarial anaemia and cerebral malaria in mouse models. Dosing mice and monitoring infected mice.
- Harvesting brain, bone marrow, spleen and blood samples from infected mice and analysing the changes in erythroid and myeloid cells using flow cytometry.
- Analysing proliferation and differentiation of mouse hematopoietic stem cell colony forming units in malarial infection using cell culture.
- Creating SOPs, data analysis, preparing reagents and maintaining inventory.
- Description: studying the effects of anaemia and cerebral malaria in mouse model of malaria.
AI Generalist
Outlier
San Fransesco, USA
05.2025 - Current
- Analyzed operational data to identify areas for improvement and increased efficiency.
AI Generalist
Outlier
San Francisco, CA
05.2025 - Current
- Adopting a “user mindset” to produce natural data to meet the realistic needs for using Artificial intelligence.
- Using the tool of rubrics to address user needs in a structured way.
- Evaluating AI outputs by reviewing and ranking reasoning and problem-solving responses from large language models.
- Contributing across projects depending on your specific skillset and experience.
Quality assurance analyst in PNS Non IVV products
Seqirus, CSL
Parkville Victoria, Australia
10.2019 - 10.2021
- Performing microbiological and immunological bench assays, testing the potency of anti-venoms and anti-toxins.
- Calibration of lab equipment and reagent preparation.
- Animal monitoring and dosing (mice and guinea pigs).
- Proficient in GMP, GDP practices, LIMS, GLIMS and statistical software such as Combistats and Graphpad, data entry and report writing.
Teacher Volunteer
KidZee Primary School
Nellore, AP, India
04.2011 - 03.2012
- Developed engaging lesson plans aligning with curriculum standards.
- Implemented differentiated instruction to accommodate diverse learning styles.
- Fostered positive classroom environment promoting student engagement and collaboration.
- Assessed student progress through formative and summative evaluations, providing timely feedback.
- Prepared quizzes, tests and examinations to gauge how well students were learning.
- Fostered student curiosity and interest through creative hands-on activities.
- Modified lessons and curriculum to accommodate diverse learners by using strategies such as peer-assisted learning and group work.
- Developed and implemented classroom routines to address varying student needs.
- Improved student engagement by incorporating interactive learning technologies into lesson plans.
Research specialist
University of North Carolina
Chapel Hill, NC
02.2007 - 11.2009
- Expression and purification of recombinant proteins (Cl2, alpha synuclein, FlgM, Cytochrome C) from bacteria and insect cell cultures.
- Purification of proteins using FPLC (fast protein liquid chromatography).
- Worked as lab manager for a lab of 20 students, general lab maintenance such as ordering supplies, making reagents and creating SOPs.
- Description: studying the effect of recombinant proteins expressed and purified from E.coli and insect cell cultures in protein crowding during Alzheimer's and Parkinsonism.
Clinical research assistant
Sri Venkateswara Institute of Medical Sciences
Tirupati, Andhra Pradesh, India
06.2002 - 06.2003
- Screening and recruiting patients with type II diabetes for the study.
- Analysing serum insulin levels using radio immuno assay and studying the effects of calcium and vitamin D levels on the reversal of fluorosis.
- Description: Studying combined effects of chromium and Glipizide on insulin levels in type II diabetics.
Education
Certificate - Principles of GCP
Praxis Australia
PhD - School of Medicine
Deakin University
Waurn Ponds, Geelong, Victoria08.2017
Post graduate diploma - industrial bioinformatics by distance Education
Bioinformatics institute of India
Noida, Uttar Pradesh, India05.2012
Master of Science - Forest Biotechnology and Molecular Biology
Michigan Technological University
04.2006
Skills
- Molecular biology techniques: cloning, protoplast isolation, bacterial transformations, PCR, DNA sequencing, gel electrophoresis
- Microbiology: Bacterial transformations, maintaining aseptic cultures and observing cultures under microscope
- Cell Culture: Aseptic culture techniques, maintenance of cell cultures such as methyl cellulose culture of bone marrow cells, maintaining mammalian cell lines
- Hands-on flow cytometry expertise
- Skilled in dosing and monitoring laboratory animals
- Biochemistry: Expression and purification of recombinant proteins, purification of proteins using FPLC, western blotting, SDS PAGE
- Bioinformatics: GCG, EMBL, JGI and other web-based sequence analysis Biological data bases and their management, computational biology, bio Perl, bio-java, C, bio statistics
- Immunology: radioimmunology assay for insulin, ELISA, bench assays to study the potency of vaccines and toxoids
- Programming: Operating systems: MS-DOS, Windows 2000/Me/Xp, Languages: C, C;
- Databases: Oracle; Tools: MS word, Exel, PowerPoint; Front end: Developer 2000;
- Statistical software and data entry: GraphPad, Combistats, LIMS, GLIMS
PUBLICATIONS
- Knowler SA, Shindler A, Wood JL, Lakkavaram A, Thomas CJ, de Koning-Ward TF, Hill-Yardin EL, Carvalho TG, Franks AE. Altered gastrointestinal tract structure and microbiome following cerebral malaria infection. Parasitol Res. 2023 Jan 5. doi: 10.1007/s00436-022-07775-2. Epub ahead of print. PMID: 36602586.
- Lakkavaram A, Lundie RJ, Do H, Ward AC, de Koning-Ward TF. Acute Plasmodium berghei mouse infection elicits perturbed erythropoiesis with features that overlap with anemia of chronic disease. Front Microbiol. 2020; 11:702.
- Ghosh S, Chisholm SA, Dans M, Lakkavaram A, Kennedy K, Ralph SA, Counihan NA, de Koning-Ward TF. The cysteine protease dipeptidyl aminopeptidase 3 does not contribute to egress of Plasmodium falciparum from host red blood cells. PLoS One. 2018;13(3):e0193538.
- Li C, Charlton LM, Lakkavaram A, Seagle C, Wang G, Young GB, Macdonald JM, Pielak GJ. 2008. Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR. Journal of the American Chemical Society 130: 6310-6311.
- Poster and flash presentation: Studying malarial anemia and understanding the role of CISH in malaria. Asha Lakkavaram, Saeed Mahmoudimeimand, Alister Ward, Tania de Koning-Ward. EMBL Conference BioMalPar XI Biology and Pathology of the Malaria Parasite, Heidelberg, Germany.
- Pushpinder Brar, Asha Lakkavaram and Chandrasekhar P. Joshi: Genomic organization and expression profile of sucrose synthase genes in aspen trees. Department of Biological Sciences, Michigan Technological University.
- Divergent genes and bidirectional promoters in the rice genome. Nicholas Krom, Asha Lakkavaram, Ramakrishna Wusirika, Department of Biological Sciences, Michigan Technological University.
Languages
English
Full Professional
Hindi
Full Professional
Telugu
Full Professional
Work Availability
monday
tuesday
wednesday
thursday
friday
saturday
sunday
morning
afternoon
evening
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Accomplishments
- Took on tasks originally assigned to peers to decrease their workloads, completing them within expected time constraints during my roles as research specialist and quality assurance analyst.
- Developed detailed and well-received experimental plans, that became lab standards for the erythropoiesis study in mice during my PhD.
- Collaborated with various labs in Deakin and CSIRO in the development of cish knockout project to study various cells involved in immunity and erythropoiesis.
- Used Microsoft Excel to develop inventory tracking spreadsheets.
- Collaborated to the designing of the project by data collection and entry with accuracy and efficiency as well as data analysis.
- Documented and troubleshooted issues related to cytochrome c protein expression and purification.
- Designed flow cytometry experiments to evaluatestat5 levels of erythropoietic precursor cells by with accuracy and efficiency.
- Improved lab efficiency through effectively helping with roster duties such as lab reagent preparations, equipment validation along with duties such as ordering, lab cleaning and maintenance.
Certification
- Certificate in Principles of GCP, Praxis - 05/2019
- Certificate III in School based Education support , The Gordon TAFE - 2025-2026
- Certificate in Mentoring -Module two, EdConnect, 01/2026
- Working with Children Check - Victoria, 2027
- Mandatory reporting and other obligations for Non- Government schools, Department of Education, 11/2026
Quote
Be less curious about people and more curious about ideas.
Marie Curie
Interests
Gardening
Painting
Wood working
Movies
Software
C
Oracle
MS Office
C
GraphPad
Combistat
LIMS
GLIMS
Timeline
AI Generalist
Outlier
05.2025 - Current
AI Generalist
Outlier
05.2025 - Current
Quality assurance analyst in PNS Non IVV products
Seqirus, CSL
10.2019 - 10.2021
Lab technician
Deakin University
05.2019 - 10.2019
Lab technician
Deakin University
01.2017 - 10.2019
Teacher Volunteer
KidZee Primary School
04.2011 - 03.2012
Research specialist
University of North Carolina
02.2007 - 11.2009
Clinical research assistant
Sri Venkateswara Institute of Medical Sciences
06.2002 - 06.2003
PhD - School of Medicine
Deakin University
Post graduate diploma - industrial bioinformatics by distance Education
Bioinformatics institute of India
Master of Science - Forest Biotechnology and Molecular Biology
Michigan Technological University
Certificate - Principles of GCP
Praxis Australia