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On-SiteLocation: Macquarie Park, Australia, AUSydney, NSW
Open to relocation: Yes
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Aynal H. Rana
Macquarie Park,Australia
Work Preference
Summary
A PhD-trained Immunology Researcher specialising in human immune cell biology. Expertise in immune-cell phenotyping, functional analysis, flow cytometry, and OMICS techniques, including proteomics and glycoproteomics. Proficient in experimental design, protocol optimisation, and complex data analysis within regulated PC2 environments. Focused on advancing clinical researches through collaboration and mentorship.
Overview
3
3
Languages
5
5
Certification
14
14
years of professional experience
Work History
Animal Technician
Macquarie Animal Research Services
08.2023 - Current
- Supported researchers in preclinical studies at Macquarie University research facilities by providing technical and operational assistance.
- Liaise with researchers and facility users to coordinate project requirements, troubleshoot issues, and support efficient research delivery.
- Coordinate daily PC2 lab activities, including equipment, biological samples, consumables, inventory, documentation, and project logistics.
- Coordinated project requirements with researchers and facility users, resolving issues to enhance research delivery.
PhD Researcher
Macquarie University
10.2022 - 08.2026
- Investigated human innate immune (neutrophils, monocytes, TAMs)-cell biology and glycoimmunology across inflammatory and disease states, integrating cellular phenotyping with proteomics, glycomics and glycoproteomics.
- Isolated primary human neutrophils from healthy PBMC and designed ex vivo activation and polarisation experiments, including TNF-α stimulation and N0/N1/N2 phenotypic models to investigate immune response mechanisms.
- Performed and optimised multicolour flow cytometry for immune-cell phenotyping, including CD11b, CD62L, CD54 and CD18 surface markers, viability staining, compensation controls and flow-cytometric validation of activation/polarisation states.
- Isolated primary human neutrophils from healthy PBMC and designed ex vivo activation and polarisation experiments, including TNF-α stimulation and N0/N1/N2 phenotypic models.
- Analysed and interpreted complex multi-OMICS datasets across healthy donors, inflammatory states, septic shock, and metastatic melanoma, performing pathway/bioinformatics analyses and integrating complementary datasets to uncover insights into disease mechanisms.
- Developed and optimised experimental protocols, troubleshot technical and methodological issues, maintained comprehensive research documentation, and trained students in laboratory techniques, data analysis, and safe research practices to enhance team capability.
Project Assistant
Umeå University
10.2021 - 02.2022
- Executed mouse tissue banking, encompassing collection, embedding, cryostat/microtome sectioning, and preparation of tissue sections for analysis to facilitate research objectives.
- Formulated and refined IHC staining protocols for mouse tissue sections, enhancing microscopy analysis accuracy.
- Assisted in preclinical research through meticulous sample documentation, effective mouse colony management, and precise project budget tracking.
Visiting Scientist
Danish Cancer Society
05.2019 - 06.2019
- Banked glioblastoma (GBM) cells in a PC2 laboratory, focusing on sample processing and cryopreservation workflows.
Research Student
National Center for Cancer Immune Therapy
Herlev, Copenhagen
09.2016 - 01.2018
- Conducted translational cancer immunology research utilizing patient-derived tumour-infiltrating lymphocytes (TILs) from metastatic melanoma to compare young TILs with rapidly expanded infusion products, contributing to understanding of immune response dynamics.
- Performed multicolour flow cytometry to phenotype CD4+ and CD8+ T-cell subsets and assess differentiation, activation, and exhaustion markers, providing insights into immune cell functionality.
- Assessed functional anti-tumour responses through intracellular cytokine staining and CD107a-based flow cytometry after co-culture with autologous tumour cells, evaluating TNF-α and IFN-γ responses to determine immune efficacy.
- Analysed paired patient data to compare T-cell phenotype and tumour reactivity before and after rapid expansion and investigated associations with clinical response.
Trainee & Research Fellow
Narayana Hrudayalaya Hospital
07.2012 - 06.2014
- Executed molecular and cellular techniques, including HLA typing, stem cell processing, bone marrow analysis, and CD34+ cell analysis by flow cytometry to support research objectives.
- Facilitated daily laboratory operations by overseeing biological sample processing, ensuring precise documentation, and managing consumables and inventory.
- Maintained accurate sample and experimental records while effectively communicating laboratory results to clinical and technical teams.
Education
Doctor of Philosophy (PhD) - Analytical Glycoimmunology
Macquarie University
Sydney, Australia08-2026
Master of Science - Human Biology
University of Copenhagen
Copenhagen, Denmark01-2018
Skills
- Multi-omics analysis
- Flow Cytometry
- Human Immune Cell Phenotyping
- Immunology & Cancer Immunology
- Cancer immunology
- Primary neutrophil isolation
- Primary monocyte isolation
- Cell Culture
- Experimental design
- Analytical techniques
- Laboratory Operations Management
- Data documentation
- Student Training
Certification
- ICH-GCP, Western Australian Health Translation Network, 12/2024
- ANZCCART ComPass, Australia, Adelaide University, 09/2023
- Approved Arrangement (AA) Accreditation for AA Accredited Persons (Classes 1-8) - Gold, Australia, 09/2023
- Swedish legislation & Ethics, animal welfare and 3R, Sweden
Umeå University, Sweden, 06/2021 - Federation of European Laboratory Animal Science Associations (FELASA), University of Copenhagen, Denmark, 05/2016
LANGUAGES
Bengali
Danish
English
Interests
Semi professional club cricketer and great interest in golfing
Publications
4 peer-reviewed publications (co-author); 2 first author manuscripts in preparation.
Timeline
Animal Technician
Macquarie Animal Research Services
08.2023 - Current
PhD Researcher
Macquarie University
10.2022 - 08.2026
Project Assistant
Umeå University
10.2021 - 02.2022
Visiting Scientist
Danish Cancer Society
05.2019 - 06.2019
Research Student
National Center for Cancer Immune Therapy
09.2016 - 01.2018
Trainee & Research Fellow
Narayana Hrudayalaya Hospital
07.2012 - 06.2014
Master of Science - Human Biology
University of Copenhagen
Doctor of Philosophy (PhD) - Analytical Glycoimmunology
Macquarie University