Bhavana Srikanth

Seeking an opportunity to contribute to the operations of a laboratory, where dedication to precision and adherence to protocols can support the seamless execution of experiments and analyses. Eager to be part of a team working towards scientific advancements, aiming to ensure the efficient functioning of the laboratory and assist in maintaining a high standard of research and experimentation.

• Laboratory Techniques: - Bacterial cloning and transformation - DNA/RNA/Protein/Glycan extraction and purification - Real-time PCR, Gradient PCR, Colony PCR - Gel Electrophoresis, SDS-PAGE - Fluorometric analysis
• Analytical Chemistry: - Titration - Gas Chromatography-Mass Spectrometry (GCMS) - Liquid Chromatography-Mass Spectrometry (LCMS) - High-Performance Liquid Chromatography (HPLC) - Wet analysis methods
• Computational Analysis: - Sequence alignment tools: NCBI-BLAST, Mega X - Image analysis using Image J software - Statistical Analysis with Prism Graph Pad - Protein analysis tools: MaxQuant-LFQ, ExPASy, PyMOL, UniProt, PDB - Glycan Analysis with Glycomod
• Computer Proficiency: - Microsoft Word, Excel, PowerPoint
• Microbiology Expertise: - Manual and Automated Blood culture systems - Routine culture and sensitivity of specimens (exudates and body fluids, blood, CSF, respiratory samples, urine, bile, and stool) - BACTEC, VITEK - Differential staining - Routine processing of various specimens in Mycology - Identification and isolation of clinically significant fungi by LPCB, Calcofluor white mount - Culture method and screening of occult blood in stool specimens in Parasitology
• Serological Diagnostics: - Infectious agents detection by agglutination and precipitation techniques (Widal test, Weil-Felix test) - Enzyme-Linked Immunosorbent Assay (ELISA)

Expression and purification of recombinant α-l-fucosidase, AlfC protein. The Australian Research Council Training Centre for Facilitated Advancement of Australia's Bioactives (FAAB)- Macquarie University. (NATA Accredited) December 2023- February 2024.

1. Preparation of cell cultures

2.Recombinant protein production

3. Protein expression and purification

4. In vitro enzyme activity assay

5. Identification of glycan products by computational analysis

Plant Tissue Culture Laboratory, GKVK campus, University of Agricultural Sciences 20th September 2021 to 20th December 2021.

1. Sterilization and media preparation

2. Isolation of desired explant

3. Preparation of tissues for culturing (Initiation)

4. Subculturing (Multiplication)

5. Observation of tissue-cultured plants for contaminants and growth

6. Primary and secondary hardening of tissue-cultured plants.

Vikram Hospital (Bengaluru) Private Limited 14th December 2020 - 15th February 2021.

1. Serology-(ELISA method).

2. WIDAL test

3. Leishman staining for malarial testing. Assisted during Phlebotomy and inoculation of Bacterial cultures in a microbiology laboratory in aseptic conditions.

4. Biochemical tests for identification of different microorganisms

5. Gram's Staining

6. Antibiotic susceptibility testing

7. Weil's Felix test for confirming Rickettsia fever.

8. Urine Analysis & Stool test

9. Complete Blood test

10. Histopathological techniques- Grossing, Tissue Processing, Embedding, Microtome sectioning,

11. Frozen sectioning, Mounting, Staining (H & E stain, PAS Stain & PAP Stain).

• Biosafety Awareness (Macquarie University, 2023)
• Gene Technology (Macquarie University, 2023)
• Laboratory Health and Safety induction (Macquarie University, 2023)
• Hazardous Chemical Handling (Macquarie University, 2023)
• Lab Essentials (Macquarie University, 2023)
• Lazer Safety (Macquarie University, 2023)
• CPR and First Aid (Royal Life Saving, NSW, October, 2023)

• Biosafety Awareness ( Macquarie University, 2023 )
• Gene Technology ( Macquarie University, 2023 )
• Laboratory Health and Safety induction ( Macquarie University, 2023 )
• Hazardous Chemical Handling ( Macquarie University, 2023 )
• Lab Essentials ( Macquarie University, 2023 )
• Lazer Safety ( Macquarie University, 2023 )
• CPR and First Aid (Royal Life Saving, NSW, October,2023).

• National Level Swimmer and Water Polo Medallist (Junior & Senior level), conducted by The Swimming Federation of India.
• 1st place in Creation of Miniature Garden for Sustainable Environment conducted by Ramaiah College of Arts, Science & Commerce during National Science Day.

• Dr Rachael Dudaniec, Senior Lecturer, School of Biological Sciences, Macquarie University, 0450615055, rachael.dudaniec@mq.edu.au
• Dr Vijayaprabah Maheswaran, Family Medical Clinic, 0480298184