Summary
Overview
Work History
Education
Skills
Accomplishments
Published Projects
References
Timeline
Generic

Bindu Kshetri Bhattarai

Brooklyn Park,SA

Summary

Motivated and detail-oriented biotechnology graduate with strong experience in pharmaceutical quality control and laboratory research. Proficient in a range of laboratory techniques, including HPLC analysis, UV-Vis spectrophotometry, murine cell culture, XTT and Sytox Green assays, and microbiological assays. Skilled in reagent preparation, sample handling, maintaining laboratory safety, and ensuring compliance with SOPs, and GMP standards. Efficient in data analysis and safe lab practices, ensuring accurate and reliable results. Consistently demonstrates a strong work ethic, contributing to successful project outcomes, and maintaining high standards in laboratory operations.

Overview

8
8
years of professional experience

Work History

Postgraduate Research

Flinders University
02.2024 - 11.2024
  • Conducted research studies focusing on evaluating the effects of urinary tract analgesics on bladder cancer cells in vitro.
  • Proficient in maintaining MB49 murine bladder cancer cells.
  • Conducted XTT assays to evaluate metabolic activity and assess the cytotoxic effects of compounds on MB49 bladder cancer cells, demonstrating proficiency in cell viability assessment techniques.
  • Utilized Sytox Green staining to determine cell viability and apoptosis, ensuring accurate measurement of cytotoxic responses.
  • Monitored cell proliferation and morphology using the Incucyte Live-Cell Imaging System, enabling real-time analysis of cellular responses to treatments.
  • Utilized GraphPad Prism for statistical analysis including ANOVA, t-tests, and data visualization (bar graphs, scatter plots, error bars).
  • Collaborated with supervisors in experimental design, data interpretation and troubleshooting laboratory challenges.
  • Presented findings through progress reports, presentations, and academic discussions within the research group.

Quality Control Officer

Time Pharmaceuticals Pvt. Ltd
Nawalparasi, Nepal
01.2019 - 01.2023
  • Prepared reagents and test samples for raw materials, in-process samples, and finished pharmaceutical products, in line with SOPs and GMP requirements.
  • Operated analytical instruments, including HPLC, dissolution testers, disintegration apparatus, and UV-Vis spectrophotometers.
  • Recorded and maintained accurate data and laboratory documentation in line with GMP and audit requirements.
  • Followed detailed protocols to ensure consistency, accuracy, and regulatory compliance.
  • Conducted stability studies and related substances testing to assess product quality, shelf life, and compliance with pharmacopeial standards.
  • Calibrated laboratory equipment regularly to ensure accuracy in all test results.
  • Monitored laboratory stock, maintained equipment logs, and ensured readiness for internal and external audits.

Research Student

Narayani Samudayik Hospital
Chitwan, Nepal
06.2016 - 12.2017
  • Conducted research studies focusing on the prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from pus samples in collaboration with Narayani Samudayik Hospital, Chitwan, Nepal.
  • Performed microbiological techniques including culturing of pus samples, Gram staining, and sub-culturing for bacterial isolation.
  • Carried out biochemical identification tests (catalase, coagulase, and Mannitol Salt Agar fermentation) to confirm Staphylococcus aureus.
  • Performed antibiotic susceptibility testing (AST) to evaluate resistance patterns, focusing on methicillin resistance.
  • Recorded, organized, and analyzed experimental data to determine prevalence rates of MRSA in clinical samples.
  • Prepared detailed thesis report and presented findings to academic supervisors.

Education

Master of Biotechnology -

Flinders University
Australia
11-2024

Master of Microbiology -

Tribhuvan University
Nepal
10-2018

Skills

  • HPLC, UV-Vis, dissolution & disintegration testing
  • Reagent & sample preparation
  • Murine cell culture (MB49)
  • XTT and Sytox Green assays, IncuCyte imaging
  • Microbiological culture and antibiotic susceptibility (MRSA)
  • Data analysis and visualization (GraphPad Prism)
  • GMP documentation and record keeping
  • Equipment calibration
  • Quick adaptation to new laboratory technologies
  • Time management and attention to detail
  • Strong communication skills between team members and supervisors

Accomplishments

  • Chancellor’s commendation for high GPA, Flinders University, 2024

Published Projects

Prevalence of methicillin-resistant Staphylococcus aureus isolated from clinical samples at Narayani Samudayik Hospital, Chitwan, Nepal, Adhikari, R., Kshetri, B., Sharma, K. N., Khanal, S., Panta, O. P Sharma, S Poudel, Tribhuvan University Journal of Microbiology, 7, 37-42, 2020, https://doi.org/10.3126/tujm.v7i0.33794

References

Dr Luke Grundy

Ralph Ernst, Senior Research Fellow in Prostate/Bladder Cancer

College of Medicine and Public Health, Flinders University

Luke.grundy@flinders.edu.au

Dr. Pramod Poudel

Associate professor

Central Department of Biotechnology, Tribhuvan University, Nepal

pramod.poudel@cdbt.tu.edu.np

Timeline

Postgraduate Research

Flinders University
02.2024 - 11.2024

Quality Control Officer

Time Pharmaceuticals Pvt. Ltd
01.2019 - 01.2023

Research Student

Narayani Samudayik Hospital
06.2016 - 12.2017

Master of Biotechnology -

Flinders University

Master of Microbiology -

Tribhuvan University
Bindu Kshetri Bhattarai