

I am an accomplished scientist with over 10 years of experience in biological and biomedical research laboratories and 2 years in diagnostic laboratories. I am skilled in molecular and immunology techniques following SOPs, regulatory compliance, and GLP guidelines. I have a proven ability to enhance laboratory efficiency through meticulous inventory management, problem-solving, and strong analytical skills. I can maintain a conscientious attitude in a laboratory by following health and safety protocols and utilising my knowledge of lab procedures to advance projects with rapidly changing priorities and timelines. Passionate about collaborative research and committed to achieving impactful results through critical thinking, communication, and teamwork.
Cell Biology & Tissue Culture
PBMC Isolation
Haematopoietic Progenitor Cell Culture
Bone Marrow Aspiration
Explant and Cell Lines Culture
Primary and Stem Cell Culture
Cryopreservation
Immunology & Immunoassays
Immunocytochemistry
Immunohistochemistry
ELISA
RIA
Direct Fluorescent Antibody Tests
Indirect Fluorescent Antibody Tests
Serum Agglutination Test
Complement Fixation Test
Double/Triple Staining
Full Blood Count
Drug Treatment Fluorescent Plate Reader-Based Functional Assays
Flow Cytometry & Related Assays
Flow Cytometry
FACS
Cell Apoptosis Analysis
Cell Proliferation and Cell Cycle Assay
Cell Viability Assay
Cell Migration Assay
Ca²⁺Flux Assay
Microscopy
Light Microscopy
Fluorescent Microscopy
Molecular Biology & Genomic Techniques
DNA extraction and purification (manual and automated)
PCR setup and amplification
Sample preparation for sequencing and genotyping
DNA quantification and quality assessment
Specimen Reception & Accessioning
Specimen receipt, verification, and accessioning
Laboratory Information Management Systems (LIMS)
Sample traceability and patient identification
Specimen quality assessment and discrepancy reporting
Accurate laboratory record keeping
Molecular Diagnostics & Instrumentation
PCR instrument operation and monitoring
Spectrophotometer and centrifuge operation
Automated DNA extraction systems
Liquid handling and robotic run setup
Equipment calibration, maintenance, and troubleshooting
Data Analysis & Software Proficiency
Statistical Analysis: GraphPad Prism, Sigma Plot, SPSS, Microsoft Office
Bioinformatics Tools: Geneious, NCBI, GenBank, Vector NTI
Laboratory Information Management: STARLIMS
Photoshop, EndNote
Laboratory Operations & Safety
Laboratory organisation and biosafety compliance
Hazardous material handling and waste disposal
Inventory and reagent management
Laboratory cleanliness and workflow optimisation
Quality Assurance & Regulatory Compliance
Good Laboratory Practice (GLP)
ISO 15189 compliance
NATA/ISO accreditation standards
Quality control checks and internal audits
Professional & Cognitive Skills
Attention to detail and analytical thinking
Problem-solving and troubleshooting
Effective communication and teamwork
Strong written and verbal communication skills
Time management and multitasking
https://scholar.google.com/citations?user=oOigSdEAAAAJ&hl=en
List of Selected Publications:
· Kamal, T., Green, T. N., Hearn, J. I., Josefsson, E. C., Morel-Kopp, M. C., Ward, C. M., During, M. J., & Kalev-Zylinska, M. L. (2018). N-methyl-D-aspartate receptor-mediated calcium influx supports in vitro differentiation of normal mouse megakaryocytes but proliferation of leukemic cell lines. Research and Practice in Thrombosis and Haemostasis, 2(1), 125–138.
· Khan, L. B., Swift, S., Kamal, T., & Read, H. M. (2018). Simulation of MICROBACT strip assay using colored liquids to demonstrate identification of unknown Gram-negative organisms in undergraduate laboratory. Journal of Microbiology & Biology Education, 19(2).
· Kamal, T., Green, T. N., Morel-Kopp, M. C., Ward, C. M., McGregor, A. L., McGlashan, S. R., Bohlander, S. K., Browett, P. J., Teague, L., During, M. J., Skerry, T. M., Josefsson, E. C., & Kalev-Zylinska, M. L. (2015). Inhibition of glutamate-regulated calcium entry into leukemic megakaryoblasts reduces cell proliferation and supports differentiation. Cellular Signalling, 27(9), 1860–1872.
· Kalev-Zylinska, M. L., Kamal, T., Green, T. N., Morel-Kopp, M. C., Baguley, B., et al. (2013). Megakaryocyte regulatory pathway mediated by NMDA receptors. Journal of Thrombosis and Haemostasis, 11, 780.
· Howie, G. J., Sloboda, D. M., Kamal, T.,& Vickers, M. H. (2009). Maternal nutritional history predicts obesity in adult offspring independent of postnatal diet. The Journal of Physiology, 587(4), 905–915.
· Howie, G., Sloboda, D. M., Kamal, T., & Vickers, M. H. (2009). A maternal high-fat diet either preconceptionally and/or throughout pregnancy and lactation leads to obesity in offspring independent of postnatal diet. The Journal of Physiology, 587, 905–915.
· Kim, B. K., Kang, S. R., Jabed, M. A., Kim, D. E., Han, C. H., Huh, M. K., & Kamal, T. (2008). Effects of spermatozoa during in vitro meiosis progression in porcine germinal vesicle oocytes. Animal Reproduction Science, 104(1), 83–92.
Conference Proceedings
· Kamal, T., Green, T. N., Morel-Kopp, M. C., Ward, C. M., Skerry, T. M., Josefsson, E. C., & Kalev-Zylinska, M. L. (2015). A novel mechanism of calcium entry that increases growth of leukemic megakaryoblasts. 5th Asia-Pacific Summit on Cancer Therapy, Brisbane, Australia.
Best Poster Award - Recognised for research quality, novelty, and significance.
· Kamal, T., Green, T. N., Morel-Kopp, M. C., Ward, C. M., Skerry, T. M., Josefsson, E. C., & Kalev-Zylinska, M. L. (2015). Impact of glutamate-mediated calcium pathways on the growth of leukemic megakaryoblasts. Gordon Research Conference on the Cell Biology of Megakaryocytes and Platelets, Lucca (Barga), Italy.
· Kamal, T., Green, T. N., Morel-Kopp, M. C., Ward, C. M., During, M. J., Teague, L., Browett, P. J., & Kalev-Zylinska, M. L. (2014). Can glutamate pathways influence neoplastic megakaryopoiesis? New Directions in Leukaemia Research Conference, Noosa, Queensland, Australia.
· Kamal, T., Green, T. N., Leung, E., McGregor, A. L., Baguley, B., & Kalev-Zylinska, M. L. (2013). Glutamate pathways appear functional in human megakaryocytic cells. Medical Sciences Congress, Queenstown, New Zealand.
· Kamal, T., Green, T. N., Morel-Kopp, M. C., Teague, L., Browett, P. J., During, M. J., & Kalev-Zylinska, M. L. (2013). Megakaryocyte regulatory pathway mediated by NMDA receptors. XXIV Congress of the International Society on Thrombosis and Haemostasis (ISTH), Amsterdam, Netherlands.
· Green, T. N., Kamal, T., Chugh, S., Leung, E., Baguley, B., Morel-Kopp, M. C., Ward, C. M., & Kalev-Zylinska, M. L. (2012). NMDA receptors in neoplastic megakaryocytes. Haematology Society of Australia and New Zealand Annual Meeting, Melbourne, Australia.
· Sloboda, D. M., Kamal, T., Green, M. P., Howie, G. C., & Vickers, M. H. (2008). Maternal high-fat nutrition leads to early-onset puberty, postnatal obesity and alterations in sex hormone profiles in adult offspring. Endocrine Society Annual Meeting, 2008.
· 2022 – Collaborative Institutional Training Initiative (CITI Program), St. Jude Children's Research Hospital
Completed the following modules:
- Human Subjects Research – Biomedical, Basic Course
- Good Clinical Practice (GCP) for Clinical Trials with Investigational Drugs and Medical Devices (U.S. FDA)
· 2022 – Certificate of Completion, MPI Transitional and Containment Facility Training
· 2013 – Research Induction Course, School of Medicine
Topics included:
- Keys to Success with Funding
- Conducting Systematic Reviews and Meta-Analyses
- Successful Academic Publishing
- Translational Research
- Statistical Methods in Research
· Lauren Jelley, Senior Scientist, Virus Identification Reference Laboratory, Institute of Environmental Science and Research Limited (ESR), Willington, New Zealand, +64 221357100, Lauren.Jelley@esr.cri.nz
· Dr Prasanna Kumar Kallingappa, Lead Technologist, University of Auckland, Department of Molecular Medicine and Pathology, Faculty of Medical and Health Science, Auckland, 1023, New Zealand, +64 211578057, p.kallingappa@auckland.ac.nz
· 2022: The Collaborative Institutional Training Initiative (CITI Program)
I have completed the following CITI Program course under requirements set by: Basic/Refresher Course - Human Subjects Research (Curriculum Group) HSP Group 1. Bio-medical (Course Learner Group) 1 - Basic Course (Stage) - Good Clinical Practice (GCP) (Curriculum Group) Good Clinical Practice for Clinical Trials with Investigational Drugs and Medical Devices (U.S. FDA) (Course Learner Group) 1 - GCP (Stage) St. Jude Children's Research Hospital
· 2022: Certificate For the completion of MPI Transitional and Containment Facility
· 2013: Research Induction Course run by the School of Medicine
Keys include Keys to Success with Funding, Conducting Systematic Review and Meta‐Analysis, Successful Academic Publishing, Translational Research, Statistical Methods in Research, Tips for Successful Presentation.
· 2012: Polychromatic flow cytometry for the characterisation of hematopoietic and non-hematopoietic cells for clinical use Flow Cytometry Workshop