Summary
Overview
Work History
Education
Skills
Publications
Presentations
Attributes
Timeline
Generic

Evelyn Battad Bernardo

Stream Hill,Australia

Summary

A solid background in Molecular Microbiology provided myself a pathway into a fulfilling career in the health pathology sector as well as in research and academe. I have extensive work experience in the area of DNA and serology testings in the context of Infectious diseases. I also have sufficient experience in proposing and running my own research projects both in the academe and in the clinical setting. I have done University teaching abroad and in several Universities here in NSW catering for students with diverse background and learning capabilities.

Overview

20
20
years of professional experience

Work History

SESSIONAL TEACHING STAFF

UNIVERSITY OF WOLLONGONG
07.2021 - 10.2025
  • Subjects involved in : Chem 200 and Biol 214 Practical classes.July 2025 -Oct 2025
  • Biol 340 Cell and Molecular Biology Practical and tutorial classes. February 2022 to May 2024
  • Biol 103 Practical classes. July 2021 to October 2021

Resposilities:

  • Facilitated laboratory classroom discussions to enhance critical thinking and collaboration skills.
  • Demonstrated laboratory skills and techniques and/or taught laboratory skills relevant to the course.

HOSPITAL SCIENTIST

ROYAL PRINCE ALFRED HOSPITAL
11.2007 - 05.2021
  • Serology Section (part-time) & Molecular Microbiology Section
  • Department of Microbiology Infectious Diseases Group

Responsibilities and Achievements:

  • Performed and validated Hepatitis viruses, Covid-19 virus and HIV serology Ag and Ab based assays including several general serology tests assays (such as Syphillis, Dengue, CMV, HSV 1 & 2, BK virus etc.)
  • Performed and validated Hepatitis C & B qualitative & quantitative assays by PCR using the Roche Ampliprep, Taqman and Amplicor systems
  • Performed, detected and analysed different Hepatitis C genotypes
  • Performed, validated routine and more complex microbiology procedures with emphasis on Nucleic Acid detection tests
  • Helped develop the preliminary MRSA assay
  • Developed and validated the BK virus quantitative assay of the Department, which is now being used routinely
  • Developed and performed the first genotyping of BK virus detected from renal transplant patients in Sydney, which was presented at the Australian Society for Microbiology annual conference.

SCIENTIFIC OFFICER (Part-time)

DHM PATHOLOGY
01.2007 - 01.2008
  • Molecular Biology Department North Ryde, NSW

Responsibilities

  • DNA extraction, amplification and detection from urine, swabs and other human samples for the presence of Chlamydia trachomatis and Neisseria gonorrhoeae by real-time PCR technology
  • DNA extraction, amplification and detection from serum samples for the presence of Hepatitis C virus
  • Data base receipt, storage, query of specimens, including patient details and results
  • Proper maintenance, storage, and use of consumables, reagents, and equipment according to manufacturer and laboratory procedures.

HOSPITAL SCIENTIST

ST. GEORGE HOSPITAL, KOGARAH
09.2007 - 11.2007
  • Department of Microbiology
  • Duties and responsibilities very similar to those in RPAH

HOSPITAL SCIENTIST

ICPMR, WESTMEAD HOSPITAL
05.2007 - 09.2007
  • (Short contract)

Forensic Laboratory, Dept. of Analytical Laboratory

  • Processing and analysis of forensic samples to reduce the backlog of criminal cases.

SCIENTIFIC OFFICER (full-time)

DCDL PATHOLOGY
09.2006 - 12.2006
  • Microbiology Department Rhodes, NSW.

Responsibilities

  • Plate reading, analysis and identification of pathogenic microorganisms obtained from human clinical samples using morphological, biochemical and serological tests and commercial ID kits
  • Entry of results and release using the company’s LIS data base system
  • Microscopic examination of Oocytes, cysts and parasites (OCP) in stool samples
  • Gram stain preparation and analysis; antibiotic and sensitivity preparation, testing and interpretation
  • Setting up stool, urine and swab specimens

ASSOCIATE LECTURER

UNIVERSITY OF WESTERN SYDNEY
07.2005 - 08.2006

Responsibilities

  • Coordinate two units of studies in Microbiology and Biotechnology with lecture and practical components for undergraduate and postgraduate students.
  • Prepare and deliver lectures, supervise and run tutorial and practical laboratory classes
  • Undertake research and supervise post-graduate projects in collaboration with other staff members in the Department
  • Participation in administration duties as required by the School

Education

Doctor of Philosophy - Molecular Microbiology

THE UNIVERSITY OF New South Wales
01.2002

POST-GRADUATE UNIVERSITY COURSE - Microbiology

OSAKA UNIVERSITY JAPAN
01.1992

Master of Science - Food Science

UNIVERSITY OF the Philippines Los BAnos
01.1987

BACHELOR OF SCIENCE - Biology

CENTRAL LUZON STATE UNIVERSITY
01.1983

Skills

Clinical Microbiology

  • Logging in specimens
  • Setting up, plate reading and analysis of pathogenic organisms
  • Microscopy
  • Shake and batch fermentation
  • Microbial enumeration
  • Microbial ID
  • Streak and pour plating
  • Microbial sensitivity/antibiotic testing
  • Microbial storage of cultures
  • Microscopic analysis of oocytes, cysts and parasites

Serological testing

  • Covid 19 virus,Cryptosporidium, Giardia, Rotavirus, Adenovirus, Shigella species, Salmonella serotypes, BK virus, Streptococcus and Staphylococcus species
  • Hepatitis viruses (A,B,C,D,E)
  • SARS-Cov-2 and HIV serology Ag and Ab based assays

Molecular Biology

  • Real-time PCR, RT-PCR, nested PCR, inverse PCR, pan-handle PCR 16-23S
  • 16S rRNA and mitochondrial sequencing and analyses, RFLP analysis
  • Agarose/polyacrylamide gel electrophoresis
  • Cloning and recombinant DNA Tech
  • Restriction enzyme digestion
  • Subtractive, Southern/Northern Hybridisation Techniques
  • Transformation/Conjugation/Electroporation
  • Transposon/chemical/UV mutagenesis
  • Labelling non-radioactive and radioactive substances

Computer skills

  • Competence in the use of WebEx and Moodle
  • Experienced in the use of Facebook, Instagram, Youtube
  • Advanced skills in Microsoft Office suite
  • Experienced in Endnote Plus
  • Intermediate skills in Adobe Photoshop
  • Beginner in Microsoft Access
  • Extensive experience in web-based tools for sequence analysis
  • Competency in ABI computer programs

Publications

  • Sertan-de Guzman AA, Predicala RZ, Bernardo EB, Neilan BA, Elardo SP, Mangalindan GC, Tasdemir D, Ireland CM, Barraquio WL, Concepcion GP, Pseudovibrio denitrificans strain Z143-1, a heptylprodi-giosin-producing bacterium isolated from a Philippine tunicate, FEMS Microbiol Lett, 277, 2, 188-96, 2007
  • Provisionary Patent, Microorganism and the use thereof for producing heptyl prodigiosin and the use of heptyl prodigiosin, 1-2002-000516, 2002
  • Battad-Bernardo, E., S. McCrindle, I. Couperwhite and B. A. Neilan, Insertion of an E. coli lacZ gene in Acetobacter xylinus for the production of cellulose in whey, FEMS Microbiology Letters, 231, 2, 253-260, 2004
  • Bernardo, E. B., B. A. Neilan, and I. Couperwhite, Characterization, differentiation and identification of wild-type cellulose-synthesizing Acetobacter strains involved in Nata de Coco production, Systematic and Applied Microbiology, 21, 599-608, 1998
  • Provisionary Patent, The microorganism and the processes thereof in generating the mutant Acetobacter xylinus for the production of cellulose in lactose, 1998

Presentations

  • Battad-Bernardo, E, R. Sinha, B.A. Neilan and R. Chan, Preliminary Genotyping of BK Virus Detected from Renal Transplant Patients in Sydney, Australian Society for Microbiology Inc. Annual Scientific Convention, 07/04/11, Hotel Grand Chancellor, Hobart, Tasmania
  • Bernardo, EB, S. Dubedat, G. Mckew, and R. Chan, A BK virus quantitation assay in renal transplant recipients: development, validation and clinical application, Australian Society for Microbiology Annual Scientific Conference, 07/01/10, Sydney Convention Centre, Sydney, Australia
  • Battad-Bernardo, E., B. A. Neilan, I. Couperwhite, Cellulose biosynthesis: metabolism, genetics and regulation in two Acetobacter species, Australian Society for Microbiology, Inc. Beckton and Dickenson Award, 07/01/01, BD Bldg, North Ryde, NSW
  • Bernardo, E.B., S. McCrindle, B. A. Neilan, and I. Couperwhite, A lactose-utilizing Acetobacter strain for the production of cellulose in whey, IXth International Congress of Bacteriology and Applied Microbiology (IUMS), 08/16/99, Sydney Convention Center, Darling Harbour, Sydney, Australia

Attributes

  • Strong Attention to details
  • Laser focused on the work at hand
  • Hard working, very independent and works well in a team environment
  • Very organised and meet deadlines
  • Ability to manage time effectively and efficiently
  • Honest, respectful, friendly and able to resolve conflicts in the work place
  • Willing to go the extra mile be it overtime, shift work or weekend rosters
  • Effective strong communication skills both written and verbal

Timeline

SESSIONAL TEACHING STAFF

UNIVERSITY OF WOLLONGONG
07.2021 - 10.2025

HOSPITAL SCIENTIST

ROYAL PRINCE ALFRED HOSPITAL
11.2007 - 05.2021

HOSPITAL SCIENTIST

ST. GEORGE HOSPITAL, KOGARAH
09.2007 - 11.2007

HOSPITAL SCIENTIST

ICPMR, WESTMEAD HOSPITAL
05.2007 - 09.2007

SCIENTIFIC OFFICER (Part-time)

DHM PATHOLOGY
01.2007 - 01.2008

SCIENTIFIC OFFICER (full-time)

DCDL PATHOLOGY
09.2006 - 12.2006

ASSOCIATE LECTURER

UNIVERSITY OF WESTERN SYDNEY
07.2005 - 08.2006

Doctor of Philosophy - Molecular Microbiology

THE UNIVERSITY OF New South Wales

POST-GRADUATE UNIVERSITY COURSE - Microbiology

OSAKA UNIVERSITY JAPAN

Master of Science - Food Science

UNIVERSITY OF the Philippines Los BAnos

BACHELOR OF SCIENCE - Biology

CENTRAL LUZON STATE UNIVERSITY
Evelyn Battad Bernardo