Feng Liang

• Provided daily care for laboratory rats and mice, including feeding, watering, and health monitoring, ensuring optimal animal welfare.

• Maintained clean and sanitary housing environments through regular cage changing and cleaning.

• Observed animals for signs of illness or injury, promptly reporting any concerns to the supervising veterinarian or senior technician.

• Prepared and maintained equipment and supplies necessary for animal care and research activities.

• Assisted researchers and veterinary staff with experimental procedures, contributing to the success of research projects.

• Conducted sampling of Spinibarbus caldwelli, DNA extraction, PCR experiments, and microsatellite data analysis in the project "Assessing the Effectiveness of Fish Stocking in Huyang Creek Using Microsatellite Technology." Contributed significantly to the writing of related research articles.

• Collected water sampling and data analysis efforts in the research of Jiulong River environmental DNA detection, and authored comprehensive research articles documenting the findings.

• Designed and executed animal behaviour experiments in the study of the relationship between physiological structure and motor function in Cromileptes altivelis.

• Improved experiment design, collected data, and analyzed results in the study of the behaviour of Amphiprion clarkii growth and its potential impact on sex differentiation.

• Conducted squid anatomy, sample collection, DNA extraction, and qPCR in the study on the statolith of squid, ensuring precise molecular experiment execution.

• Performed cell culture tasks including cell resuscitation, media exchange, passaging, and cryopreservation, ensuring optimal growth conditions and cell viability.

• Conducted cell experiments such as scratch assays, in vitro cell migration and invasion assays, EdU assays, reactive oxygen species (ROS) assays, and cell proliferation assays (CCK-8), contributing to comprehensive cell behavior analyses.

• Executed molecular experiments, including Western blotting (WB), quantitative polymerase chain reaction (qPCR), and enzyme-linked immunosorbent assay (ELISA), to analyze protein expression and gene activity.

• Managed daily care for laboratory rats and mice, including feeding, temperature control, and cage cleaning, maintaining a controlled and healthy environment for animal studies.