Hamidreza Sadegh

Research Biologist offering expertise in animal experiments, molecular biology and laboratory management experiences. Hardworking and passionate job seeker with strong organizational skills eager to secure entry-level Researcher position. Ready to help team achieve company goals.

Research fellow, Griffith University, 07/2023

• Sadegh H*, Choi J, Hansbro N.G, Hansbro P, Investigation of the role of the gut microbiome in the progression of Cerebral cavernous malformation (CCM) on experimental mice model. (In preparation)

• Kamakoli MK*, Sadegh H*, Farmanfarmaei G, Masoumi M, Fateh A, Javadi G, Jamnani FR, Vaziri F, Siadat SD. Evaluation of the impact of polyclonal infection and heteroresistance on treatment of tuberculosis patients. Scientific Reports. 2017; 7:41410.
• *These authors contributed equally to this work.

• Sadegh H*, Kamakoli MK*, Farmanfarmaei GH, Masoumi M, Abdolrahimi F, Fateh A, Ebrahimzadeh N, Jamnani FR, Vaziri F, Siadat SD. Pros and cons of direct genotyping on tuberculosis clinical samples. Microbial Pathogenesis. 2017 Feb 28; 103:135-8.
• *These authors contributed equally to this work.

• Farmanfarmaei G, Kamakoli MK, Sadegh H, Masoumi M, Abdolrahimi F, Fateh A, Ebrahimzadeh N, Javadi G, Jamnani FR, Vaziri F, Siadat SD. Bias in detection of Mycobacterium tuberculosis polyclonal infection: Use clinical samples or cultures. Molecular and Cellular Probes. 2017 Jan 24.

• Yari H, Ganjalikhany MR, Sadegh H. In silico investigation of new binding pocket for mitogen activated kinase kinase (MEK): Development of new promising inhibitors. Computational Biology and Chemistry. 2015 Dec 31; 59:185-98.

• Sadegh H, Yari H, Hamid RM, Mohebali N, Ganjalikhany MR. Study of directed insilico mutations on polyphenol oxidase to improve enzyme–substrate binding efficiency. Clinical Biochemistry. 2011 Sep 30; 44(13): S103.

• 14th international conference of Cerebral Vascular Biology (CVB), Uppsala, Sweden, 06/18/2023, 06/22/2023, Rapid oral presentation and poster, Preventing stroke from cerebral cavernous malformations by targeting the microbiome
• 17th international Iranian Congress of Microbiology, Tehran, Iran, 08/23/2016, 08/25/2016, Oral presentation, Genotyping of Mycobacterium Tuberculosis strains directely on clinical samples
• 13th Conference on Biophysical Chemistry (CBC), Ardabil, Iran, 08/16/2015, 08/28/2015, Poster presentation, Investigation of new binding pocket for Mitogen Activated Protein Kinase Kinase
• 4th International Conference of Biochemistry and Molecular Biology, Mashhad, Iran, 09/06/2011, 09/09/2011, E-Poster presentation, Study of directed insilico mutations on polyphenol oxidase to improve enzyme-substrate efficiency

• Experimental animal work (Mice)
• Mice restrain and health monitoring
• Euthanasia with CO2
• Setting up breeding pairs
• Drug injection (igas, ip, oral gavage)
• Faecal matter transplantation
• Diet intervention
• Mice dissection and tissue collection (brain, gut, liver,...)
• Ear tagging and genotyping
• Molecular analysis
• PCR (qPCR, Gradient PCR, RT-PCR, Multi-PCR)
• Protein Blotting (western blot, eastern blot)
• LPS quantification
• DNA extraction (tissues, stool, bacteria)
• RNA extraction (tissues)
• Enzyme-linked immunosorbent assay (ELISA)
• Spoligotyping (MTBC)
• Histological analysis
• Tissue processing and paraffin embedding
• Tissue sectioning (Microtome, Cryostat)
• Histological staining (H&E, ab-PAS, Giemsa, Silver)
• Immunohistochemistry (IHC)
• Immunofluorescence (IF)

• Professor Philip Hansbro, Chair of Inflammation, Faculty of Science, University of Technology Sydney (UTS), +61 - 427263084, Philip.Hansbro@uts.edu.au
• Associate Prof. Danielle Stanisic, Principal Research Fellow, Institute for Glycomics, Malaria Research Group, Griffith University, +61 - 434120142, d.stanisic@griffith.edu.au
• Dr Peter Choi, Leader of Cardiovascular and Stroke Program, Centre for Inflammation, Faculty of Science, University of Technology Sydney (UTS), +61 – 410588161, jaesung.choi@uts.edu.au
• Dr Nicole Hansbro, Senior Research Fellow – Lab Manager, Centre for Inflammation, Faculty of Science, University of Technology Sydney (UTS), +61 – 427569807, nicole.hansbro@uts.edu.au

Institute of Glycomics, Griffith University