HARIKRISHNAN PARAPPALLIYALIL
North Melbourne
Summary
Experienced Biochemist with extensive expertise in the design, development, and optimization of molecular diagnostic tests. Proven track record in gene sequence analysis, assay development, and clinical validation studies. Skilled in supervising research activities, ensuring regulatory compliance, and maintaining high standards of laboratory safety. Committed to advancing diagnostic technologies to improve clinical care and public health outcomes.
Overview
7
7
years of professional experience
Work History
Project Associate
Rajiv Gandhi Centre for Biotechnology (RGCB)
2020.11 - 2024.07
Project Title: Scientific Reinvention of Ethnic Food and Medicine from Kerala for Functional Food and Drug Development
- Investigated therapeutic potentials of plant extracts, focusing on antibacterial properties against pathogens relevant to point-of-care diagnostics.
- Evaluated and optimized antibacterial efficacy and Minimum Inhibitory Concentration (MIC) of fixed oils against key infectious agents, aligning with diagnostic test development.
- Analyzed biochemical properties and fatty acid composition using assays and GC-MS, with experience in flow cytometry, contributing to development of innovative diagnostic technologies.
Junior Research Scientist
SRH Nutritions LLP at Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST-Timed)
2018.10 - 2020.11
Project title:Development of Plant-Based Nutraceutical Formulation for Enhanced Fluid Absorption and Maintenance of Gut Health
- Developed plant-based nutraceutical formulations for enhanced fluid absorption and gut health, using electrophysiological analysis with Ussing chamber.
- Analyzed transport protein expression through molecular techniques including DNA/RNA extraction, SDS PAGE, western blotting, and PCR.
- Evaluated formulation potency through in vivo perfusion studies in Sprague Dawley rats, contributing to diagnostic test development.
- Managed hazardous and chemical inventory, maintained records for controlled substances and biological wastes, and ensured timely documentation approval.
- Supervised technicians and scientists, provided guidance, and interacted with vendors for purchasing consumables and non-consumables.
Industry Scientific Support Executive
bioMérieux, India
2018.04 - 2018.09
- Specialized in bacterial identification KITs including API (Analytical Profile Index) and Bioball (lyophilized, quantified, and standardized strain).
- Trained in premium products (Densimat, Vidas, Vitek), gaining experience in microbiology solutions to improve clinical care and enhancing skills in diagnostic testing for molecular diagnostic test design and optimization.
Biochemist
Sutharya Health Care and Diagnostic Center
2017.09 - 2018.04
- Conducted biochemical profiling of 300+ clinical samples (blood, urine, feces, semen, etc.)/day for 7 months.
- Collaborated to deliver competitive customer-focused solutions. Originated, scaled, and commercialized innovative ideas to build ground-breaking initiatives.
Project Intern
Regional Cancer Centre (RCC)
2017.04 - 2017.06
Project title - Anti-Tumor and Immunomodulatory Activity of Galactoxyloglucan-Coated Iron Oxide for Better Cancer Management
- Gained expertise in performing in vitro studies (MTT, LDH, lipid peroxidation, nitric oxide assays) on tumor cell lines (Hepa1-6, A375) and studying anti-tumor activities in the BALB/c mouse model
Education
M.Sc. Biochemistry -
Mahatma Gandhi University
Kottayam, Kerala01.2017
B.Sc. Biochemistry and Nanotechnology -
Bharathiar University
Coimbatore, Tamil Nadu01.2015
Skills
Technical Skills
- Expertise in molecular diagnostic test design, optimization, experiment design, assay development, and validation.
- Experienced working in systems with NABL ISO 15189-2012, ILAC accreditation, and NABH certification for Molecular Diagnostics; proficient in ISO/IEC 17025 standards.
- Scientific and Technical Report Documentation
- Laboratory Safety and Quality Management
- DNA, Plasmid, RNA, Protein Isolation
- cDNA Synthesis, PCR, RT-PCR, Gene sequencing
- Electrophoresis (AGE, SDS-PAGE), Western Blotting
- Protein purification, Enzyme kinetics, Biochemical assays, ELISA
- UV-Vis spectrometry, GC-MS, Flow cytometry,
In Vitro and In Vivo Research Skills:
- Cytotoxicity assays (MTT, LDH, lipid peroxidation, nitric oxide)
- Apoptosis assays (Acridine Orange/Ethidium Bromide, Hoechst)
- Small animal studies (Swiss Albino mice, Sprague Dawley rats):
-Dissection, blood, and organ collection
-Oral gavage, intraperitoneal, and subcutaneous injection
-Rat perfusion, wound healing, and loop assays
Project Management:
- Project Design and Implementation
- Supervision of Research Teams
- Coordination with Academic and Industry Partners
- Preparation of Grant Applications
Analytical Skills and Software Proficiency:
- Data Analysis and Statistical Analysis (GraphPad Prism, Origin)
- Troubleshooting and Problem Solving
- Clinical Validation Studies
- Microsoft Office (Word, Excel, PowerPoint)
- Mendeley, EndNote, NVivo, BioRender, VosViewer
- Schrödinger, DATAQ systems
Transferable Skills:
- Strong Communication Skills
- Team Leadership and Collaboration
- Attention to Detail and Time Management
- Adaptability in a Dynamic Environment
Molecularbiologytechniques
- ELISA
- DNA, Plasmid, RNA, Protein Isolation
- CDNA Synthesis
- Electrophoresis - AGE, SDS-PAGE
- PCR, RT-PCR
- Restriction Digestion
- Sonication, Electroporation, Transformation
- Induction and Overexpression of proteins
- Western Blotting
Cellculturetechniques
- Media preparation
- Cryopreservation and Reconstitution
- Determining Cell Numbers
- Passaging and maintenance of Human melanoma cell line (A375), Mice hepatoma cell line (Hepa1-6), and macrophage cell line (IC-21)
- Cytotoxicity evaluation- MTT Assay, Lactate Dehydrogenase Assay, Lipid Peroxidation Assay, Nitric Oxide Assay
- Apoptosis evaluation- Acridine orange-Ethidium Staining, Bromide Staining, Hoechst
- Cellular uptake- Perl's staining
Animalresearch
- Rat Perfusion Studies
- Loop Assay
- Stereotactic surgery (Live Demonstration)
- Dissection
- Oral Gavages, Intraperitoneal Injection, Sub-cutaneous Injection (Both Swiss Albino Mice & Sprague Dawley Rat)
- Wound healing assay on Sprague Dawley Rat
Plantbiologyrelatedanalyticalandbiochemicaltechniques
- Protein purification
- Enzyme kinetics
- Biochemical assays
- Extraction of Plant Compounds- Solvent extraction (Soxhlet apparatus), Steam distillation
- Gas Chromatography-Mass Spectrometry
- Column chromatography
- Electrophysiology
- Experienced in Electrophysiological instrument: Ussing Chamber
Microbiologytechniques
- Simple and Differential Staining
- Bacterial Motility Test
- Bacterial growth curve
- Isolation, Characterization Enumeration, of Bacteria and from Medical and Environmental samples
- Direct quantification of biofilm growth
- Fungal Identification
- Bacteriophage isolation and phage titre estimation
- Antibiotic Sensitivity Test
Research
- Rajiv Gandhi Centre for Biotechnology (RGCB), Scientific Reinvention of Ethnic Food and Medicine from Kerala for Functional Food and Drug Development, Thiruvananthapuram, Kerala, India
- SRH Nutritions LLP at SCTIMST-Timed, Development of Formulations for Enhanced Absorption and Maintenance of Gut Health, Thiruvananthapuram, Kerala, India
- BioMérieux, India Private Limited, Experience: Expertise in handling Bioball and API Kits, Delhi, India
- Molecular Medicine Research Division, Regional Cancer Centre, Anti-Tumor and Immunomodulatory Activity of Galactoxyloglucan Coated Iron Oxide Nanoparticles for Better Management of Cancer, Kerala, India
- Sevana Hospital, Biochemical profiling of various clinical samples (blood, hormones, tissue, urine, etc.), Pattambi, Kerala, India
Internationalconference
Archana S., Anish N.P., Manoj P., Arya A., Sethi S N, Harikrishnan P. 2023. Scientific Validation of an Ethnoveterinary Formulation Used for Wound Healing. Proceeding of the 22nd International Bioresource Conclave and Ethnopharmacology Congress. Page 62. International Society for Ethnopharmacology. https://isesfec2023.in/ibsd-sfe/web/download
Personal Information
Date of Birth: 02/05/95
Publications
Extraction of fixed oil from wild oil-yielding plants and subsequent exploration of their physicochemical properties and biological activities. (Under communication)
References
- Dr. Sreelekha T.T, Associate Professor, Laboratory of Biopharmaceuticals & Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India, ttsreelekha@gmail.com
- Dr. S. Asha Nair, Scientist - G, Cancer Research, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India, sasha@rgcb.res.in
- Dr. Anish N P, Scientist - C, Rajiv Gandhi Centre for Biotechnology, Department of Biotechnology Government of India, Thiruvananthapuram, Kerala, India, anishnp@rgcb.res.in
Timeline
Project Associate
Rajiv Gandhi Centre for Biotechnology (RGCB)
2020.11 - 2024.07
Junior Research Scientist
SRH Nutritions LLP at Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST-Timed)
2018.10 - 2020.11
Industry Scientific Support Executive
bioMérieux, India
2018.04 - 2018.09
Biochemist
Sutharya Health Care and Diagnostic Center
2017.09 - 2018.04
Project Intern
Regional Cancer Centre (RCC)
2017.04 - 2017.06
M.Sc. Biochemistry -
Mahatma Gandhi University
B.Sc. Biochemistry and Nanotechnology -
Bharathiar University
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