Motivated and ambitious researcher, with over 7 years post-PhD experience in the field of molecular visual sciences.
Current First Aid and CPR Certification
Molecular biology: DNA, RNA & cDNA isolation, and manipulation; RACE PCR; RT-PCR; QPCR; site-directed mutagenesis; cloning; minigene construction; Next-Generation sequencing: transcriptome; gene silencing; generation of transgenic mice: conditional gene knockout and gene knockdown Zebrafish embryo micro-injection and morpholino generation
Cell culture: primary & immortalized lines; transfections; expression; cloning; infections, FACS analyses; megakaryocyte culture from bone marrow samples
Biochemistry: protein isolation; Western blotting; Immunoprecipitation
Histology: sample collection & preparation, immunohistochemistry & general staining techniques
Virology: retroviral & lentiviral production, titre, and infection of primary and immortalized cells
Whole blood handling: venesection collection, preparation, isolation of various components from both mouse and human blood
Animal handling: rodent handling, surgery, blood & organ collection; in vivo thrombosis experiments Zebrafish husbandry, sexing, spawning, embryo micro-injection, anaesthesia, and euthanasia
Platelet functional assays: aggregation, blood flow experiments; adhesion assays
Optical Coherence Tomography: retinal imaging of zebrafish larvae from as young as 3 dpf
General laboratory techniques: buffer/reagent preparation; quality control; laboratory maintenance
1. Xie, J., Jusuf, P. R., Carvalho, L. S., Davies, W. I. L. and Mountford, J. K. (2023). Comparison of visual systems in vertebrates to improve our understanding of myopia. Frontiers in Ecology and Evolution. (In Review). 2. Eilertsen, M., Davies, W. I., Patel, D., Barnes, J., Karlsen, R., Mountford, J. K., Stenkamp, D., Patel, J. S. and Helvik, J. V. (2022). An EvoDevo Study of Salmonid Visual Opsin Dynamics and Photopigment Spectral Sensitivity. Frontiers in Neuroanatomy. Provisionally accepted. 3. Nagloo, N., Mountford, J. K., Gundry, B. J., Hart, N. S., Davies, W. I. L., Collin, S, P. and Hemmi, J. M. (2022). Enhanced short-wavelength sensitivity in the blue-tongued skink, Tiliqua rugosa. J Exp. Biol. doi: https://doi.org/10.1101/2022.03.25.485754 4. Musilova, Z., Cortesi, F., Matschiner, M., Davies, W.I.L., Patel, J.S., Stieb, S.M., de Busserolles, F., Malmstrøm, M., Tørresen, O.K., Brown, C.J., Mountford, J.K., Hanel, R., Stenkamp, D.L., Jakobsen, K.S., Carleton, K.L., Jentoft, S., Marshall, J. and Salzburger, W. (2019). Vision using multiple distinct rod opsins in deep-sea fishes. Sci. 364, 6440. 5. Mountford, J.K., Davies, W.I.L., Griffiths, L.R., Yazar, S., Mackey, D.A. and Hunt, D.M. (2019). Differential stability of variant OPN1LW gene transcripts in myopic patients. Mol Vis. 25. 6. Hart, N.S., Mountford, J.K., Voight, V., Fuller-Carter, P., Barth, M., Nerbonne, J.M., Hunt, D.M. and Carvalho, L.S. (2019). The Role of the Voltage-Gated Potassium Channel Proteins Kv8.2 and Kv2.1 in Vision and Retinal Disease: Insights from the Study of Mouse Gene Knock-Out Mutations. eNeuro. 6, 1. 7. de Busserolles, F., Cortesi, F., Helvik, J.V., Davies, W.I.L., Templin, R.M., Sullivan, R.K.P., Michell, C.T., Mountford, J.K., Collin, S.P., Irigoien, X., Kaartvedt, S. and Marshall, J. (2017). Pushing the limits of photoreception in twilight conditions: The rod-like cone retina of the deep-sea pearlsides. Sci Adv. 3, 11. 8. Carvalho, L.S., Pessoa, D.M.A., Mountford, J.K., Davies, W.I.L. and Hunt, D.M. (2017). The genetic and evolutionary drives behind primate color vision. Front. Ecol. Evol. 5, 34. 9. Hart, N.S., Mountford, J.K., Davies, W.I., Collin, S.P. and Hunt, D.M. (2016). Visual pigments in a palaeognath bird, the emu Dromaius novaehollandiae: Implications for spectral sensitivity and the origin of ultraviolet vision. Proc Biol Sci. 283, 1834. 10. Petitjean, C., Setiabakti, N.M., Mountford, J.K., Aurthur, J.F., Ellis, S. and Hamilton, J.R. (2016). Combined deficiency of PI3KC2a and PI3KC2b reveals a non-redundant role for PI3KC2a in regulating mouse platelet structure and thrombus stability. Platelets. 27, 5. 11. Mountford, J.K., Petitjean, C., Putra, H.W., McCafferty, J.A., Setiabakti, N.M., Lee, H., Tønnesen, L.L., McFadyen, J.D., Schoenwaelder, S.M., Eckly, A., Gachet, C., Ellis, S., Voss, A.K., Dickins, R.A., Hamilton, J.R. and Jackson, S.P. (2015). The Class II PI 3-kinase, PI3KC2a, links platelet internal membrane structure to shear-dependent adhesive function. Nat. Comm. 6, 6535. 12. Lee, H., Sturgeon, S.A., Mountford, J.K., Jackson, S.P. and Hamilton, J.R. (2012). Safety and efficacy of targeting platelet protease-activated receptors in combination with existing anti-platelet drugs as antithrombotics in mice. Br J Pharmacol. 166, 7. 13. Hamilton, J.R., Mountford, J.K., Cornelissen, I. and Coughlin, S.R. (2009). Atherosclerosis proceeds independently of thrombin-induced platelet activation in ApoE-/- mice. Atherosclerosis. 205, 2.