Highly skilled and committed scientist with over 7 years of experience in molecular biology and use of cutting edge technology in the broad areas of hematology, oncology, reproductive biology. Self-directed and motivated with high levels of integrity and great attention to detail.
General molecular biology techniques such as plasmid, DNA, RNA preps, cDNA synthesis, quantitation, gel electrophoresis, gene cloning, expression and purification of proteins
Gene expression analysis (RNA-seq, PCR, qPCR, PAGE, protein purification, quantitation, use plate reader, western blotting and imaging using Odyssey)
Cell culture, primary cell culture, adherent, suspension culture, hanging drop culture, stem cell culture and differentiation, establishment of stable lines, proliferation, differentiation, migration assays
Gene overexpression and silencing (transfection, lentiviral and retroviral transduction) in cell lines and primary cells (umbilical cord blood hematopoietic stem cells, mouse fetal liver-derived stem cells)
Immunoprecipitation (IP/MS)/ Chromatin immunoprecipitation (ChIP-seq), Immunohistochemistry
Flowcytometry (FlowJo software), Immunofluorescence
Statistics (use of GraphPad Prism software), Bioinformatics, functional analysis of whole proteomics (LC/MS), transcriptomics (microarray and sequencing) data, animal handling, dissection, monitoring
Laboratory management, ordering, making stocks, risk assessments, ethics documents