Summary
Overview
Work History
Education
Skills
Websites
Fellowships Awards Grants
Conference Presentations
Teaching
Administrative Roles
Community Cultural Contributions
Publications
References
Training
Timeline
Generic

Karthika Radhakrishnan

Scientist
Clayton,VIC

Summary

Highly skilled and committed scientist with over 7 years of experience in molecular biology and use of cutting edge technology in the broad areas of hematology, oncology, reproductive biology. Self-directed and motivated with high levels of integrity and great attention to detail.

Overview

15
15
years of professional experience
11
11
years of post-secondary education

Work History

Adjunct Research Associate

Monash University
09.2021 - 09.2024
  • Contributed to interdisciplinary collaborations and research output by providing technical and subject matter support.

Postdoctoral Scientist

Centre for Cancer Research
6 2022 - 12.2023
  • Established in-vitro and in-vivo models to evaluate role of SNAI1 and ERG Acute Erythroleukemia disease development

Postdoctoral Scientist

Centre for Reproductive Health
05.2018 - 08.2022
  • Established role of nuclear transport protein, IPO5, in TGF-β signaling pathway and documented activin and BMP-mediated changes in gene expression program in testicular cancer cells.

Visiting Postdoctoral fellow

Tata Institute of Fundamental Research (TIFR)
07.2017 - 02.2018
  • performed gene cloning and evaluated role of several genes in early zebrafish embryogenesis

Junior Research Fellow

Rajiv Gandhi Centre for Biotechnology
08.2009 - 03.2010
  • Performed cloning, expression, purification and characterization of infertility related protein, CLP-1-4

Education

Doctor of Philosophy - reproductive health, germ cell development and epigenetics

University of Kerala
India
08.2011 - 05.2017

M.Sc. Biotechnology - molecular biology, genetics, cell biology, immunology, microbiology, tissue culture, biochemistry, bioinformatics and biostatistics

University of Mumbai
India
03.2007 - 03.2009

B.Sc. Biotechnology - molecular biology, genetics, cell biology, immunology, microbiology, tissue culture, biochemistry, bioinformatics and biostatistics

University of Mumbai
India
03.2004 - 03.2007

Skills

  • General molecular biology techniques such as plasmid, DNA, RNA preps, cDNA synthesis, quantitation, gel electrophoresis, gene cloning, expression and purification of proteins

  • Gene expression analysis (RNA-seq, PCR, qPCR, PAGE, protein purification, quantitation, use plate reader, western blotting and imaging using Odyssey)

  • Cell culture, primary cell culture, adherent, suspension culture, hanging drop culture, stem cell culture and differentiation, establishment of stable lines, proliferation, differentiation, migration assays

  • Gene overexpression and silencing (transfection, lentiviral and retroviral transduction) in cell lines and primary cells (umbilical cord blood hematopoietic stem cells, mouse fetal liver-derived stem cells)

  • Immunoprecipitation (IP/MS)/ Chromatin immunoprecipitation (ChIP-seq), Immunohistochemistry

  • Flowcytometry (FlowJo software), Immunofluorescence

  • Statistics (use of GraphPad Prism software), Bioinformatics, functional analysis of whole proteomics (LC/MS), transcriptomics (microarray and sequencing) data, animal handling, dissection, monitoring

  • Laboratory management, ordering, making stocks, risk assessments, ethics documents

Fellowships Awards Grants

  • C. Andrew Ramsden Monash Health Translation Precinct (MHTP) ECR Collaborative Award 2023 (12,000$), Hudson Institute for Medical Research
  • Recipient of Dr. Mridula Kamboj Young Scientist Award 2019, (Indian Society for the Study of Reproduction and Fertility), India
  • Recipient of Indo-US Fulbright postdoctoral fellowship (2018)
  • Tata Institute of Fundamental research (TIFR) visiting postdoctoral fellowship (2017)
  • Indian Council for Medical Research (ICMR) travel award, 2014
  • Junior research fellowship and Senior research fellowship, Council for Scientific and Industrial Research (CSIR), India (2010 to 2015)
  • Biotechnology department merit scholarship for academic excellence for Master of Science students, Mumbai university (2007-2008) and (2008-2009)

Conference Presentations

  • Karthika Radhakrishnan, Lynda Troung, Steve Lin, Jim Vadolas and Catherine Carmichael. Examining the role of EMT factor, SNAI1, in Acute Erythroid Leukemia (AEL) pathogenesis (2024), New Directions in Leukaemia Research (NDLR, Adelaide). (Poster presentation)
  • Karthika Radhakrishnan, Lynda Troung, Steve Lin, Jim Vadolas and Catherine Carmichael. Aberrantly high expression of SNAI1 altered normal erythroid differentiation and may play a critical role in Acute Erythroid Leukemia (AEL) pathogenesis. (2023), VCCC- Alliance, Melbourne Convention Centre. (Poster presentation)
  • Karthika Radhakrishnan, Michael Luu, Jessica Sutherland, Josie Iaria, Eileen McLaughin, Hong-Jian Zhu & Kate Loveland. Examining the role of Activin and BMP signaling in human testicular cancer cell lines, with emphasis on the role of nucleocytoplasmic transport protein, IPO5, in modulating their crosstalk (2022) ESA-SRB-AOTA, International Convention Centre Christchurch, New Zealand (lightening talk and finalist for best ECR poster)
  • Karthika Radhakrishnan, Julia Young, Diana Micati, Josie Iaria, Hong-Jian Zhu & Kate Loveland TGFβ signalling crosstalk during foetal germ cell development; a role for the nuclear transport protein, IPO5. (2019) ESA-SRB-AOTA, International Convention Centre Sydney, Australia. (Oral presentation)
  • Karthika Radhakrishnan & Pradeep G Kumar. Profiling the changes in the pattern of H3K4 trimethylation during the first wave of spermatogenesis in mice (2019) 29th Annual Meeting of Indian Society for the Study of Reproduction and Fertility (ISSRF), JNU, New Delhi, India. (Award talk).
  • Karthika Radhakrishnan & Kumar PG. Evaluation of the effect of over expression of AIRE, an H3K4me0 reader in germ cell line. (October 2016) International BioFest, Melbourne, Australia. (Poster presentation)
  • Karthika Radhakrishnan & Kumar PG. Evaluation of the effect of over expression of AIRE, an H3K4me0 reader in germ cell line. (October 2016) Annual Research Meeting of Epigenetics Consortium of South Australia Inc (EpiCSA), Adelaide, Australia (Poster presentation)
  • Karthika Radhakrishnan, Bhagya KP, Kumar PG. Histone methylation and Spermatogenesis-Does AIRE have a say? (Feb 2015) 25thAnnual Meeting of Indian Society for the Study of Reproduction and Fertility (ISSRF), Mumbai, India. (Poster presentation)
  • Karthika Radhakrishnan, Bhagya KP, Kumar PG. A Global Proteomic approach for elucidating the mechanism of action of AIRE in germ cells. (April 2014) 5th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE), Brisbane, Australia. (Oral presentation).
  • Karthika Radhakrishnan, Kumar PG. Histone methylation and spermatogenesis. (Feb 2013)23rdAnnual Meeting of Indian Society for the Study of Reproduction and Fertility (ISSRF), Trivandrum, India. (Poster presentation)

Teaching

Supervised MSc. students and short-term visit students

Administrative Roles

Hudson Institute Early-Mid Career Researchers (EMCR) committee member and (EMCR) grants coordinator

Community Cultural Contributions

  • Trained in Indian classical dance forms performed at prestigious events and won numerous awards and recognition
  • Recipient of Cultural talent search scholarship, Ministry of Tourism and Culture, Gov of India (1998-2001)
  • Taught Indian dance forms to children (1998 to 2008)
  • Actively participated in community welfare events and fundraising events

Publications

  • Karthika Radhakrishnan, Lynda Troung and Catherine Carmichael. An ‘unexpected’ role of EMT factors in haematological development and malignancies. Frontiers in Immunology (2023).
  • Karthika Radhakrishnan, Michael Luu, Jessie Sutherland, Eileen McLaughlin, Josie Iaria, Hong-Jian Zhu and Kate L. Loveland. Activin and BMP signaling in human testicular cancer cell lines, and a role for the nucleocytoplasmic transport protein IPO5 in their crosstalk. Cells (2023) Mar 24;12(7):1000. doi: 10.3390/cells12071000.
  • Diana J Micati, Karthika Radhakrishnan, Julia C Young, Ewa Rajpert-De Meyts, Gary R Hime, Helen E Abud, Kate L Loveland. Snail Factors in Testicular germ cell tumors and their regulation by the BMP4 signaling pathway. Andrology (2020) 8:1456–1470.
  • Kongattu P. Bhagya, Rohini J. Aswathy, Karthika Radhakrishnan, Jeeva Sengottaiyan, Pradeep G. Kumar. Autoimmune Regulator Enhanced the Expression of Caspase-3 and did not induce massive germ cell apoptosis in GC1-Spg cells. Cellular Physiology and Biochemistry (2020);54 (1):40-52.
  • A. Soumya, Karthika Radhakrishnan and Pradeep G. Kumar. DNA Methylation and Histone Modifications Associated with Male Germ Cell Differentiation. Journal of Endocrinology and Reproduction, Vol 21(2), 59-66, 2018, DOI: 10.18311/jer/2018/23323.
  • Karthika Radhakrishnan, Kongattu P Bhagya and Pradeep G Kumar. Over-expression of Autoimmune regulator in spermatogonial cell line GC1-spg altered the expression of several nucleic acid binding and cytoskeletal proteins. Molecular and Cellular Proteomics (2016) 15: 2686-2698.
  • Shreesha Sree, Karthika Radhakrishnan, Indu S, Kumar PG. Dramatic changes in 67 miRNAs during initiation of first wave of spermatogenesis in Mus musculus testis: global regulatory insights generated by miRNA-mRNA network analysis. Biology of Reproduction (2014) 91(3):1-69.

References

  • Prof. Kate L Loveland, Group Head, Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, kate.loveland@monash.edu, +61 03 8572 2904
  • Dr. Catherine Carmichael, Group Head, Leukaemia Modelling and Therapeutic Discovery group, Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, catherine.carmichael@hudson.org.au, +61 0417530347
  • Dr. Hong-Jian Zhu, Division of Medicine, dentistry and health science, Royal Melbourne Hospital, University of Melbourne, Parkville, hongjian@unimelb.edu.au, +61 0384335492

Training

  • Completed R workshop by Australia BioCommons, 2022
  • Completed training on Data organization by Monash University, 2023
  • Completed training on Animal ethics by Monash University, 2023
  • Completed ‘Introduction to Good Clinical Practices (GCP)’ training by Monash Partners, 2024

Timeline

Adjunct Research Associate

Monash University
09.2021 - 09.2024

Postdoctoral Scientist

Centre for Reproductive Health
05.2018 - 08.2022

Visiting Postdoctoral fellow

Tata Institute of Fundamental Research (TIFR)
07.2017 - 02.2018

Doctor of Philosophy - reproductive health, germ cell development and epigenetics

University of Kerala
08.2011 - 05.2017

Junior Research Fellow

Rajiv Gandhi Centre for Biotechnology
08.2009 - 03.2010

M.Sc. Biotechnology - molecular biology, genetics, cell biology, immunology, microbiology, tissue culture, biochemistry, bioinformatics and biostatistics

University of Mumbai
03.2007 - 03.2009

B.Sc. Biotechnology - molecular biology, genetics, cell biology, immunology, microbiology, tissue culture, biochemistry, bioinformatics and biostatistics

University of Mumbai
03.2004 - 03.2007

Postdoctoral Scientist

Centre for Cancer Research
6 2022 - 12.2023
Karthika RadhakrishnanScientist