Passionate about unraveling the mysteries of life through innovative research and sustainable solutions, I am a strong communicator and collaborator committed to driving scientific advancements in the field of bioscience. My expertise lies in research, project management, and biotechnological techniques, including cell culture and DNA isolation. I have a proven track record of conducting impactful research projects, such as the green synthesis of nanoparticles and studying their biological activities. Thriving in multidisciplinary teams is second nature to me. Currently pursuing a Master's in Biotechnology from the University of Melbourne, I eagerly seek opportunities to leverage my skills for innovative solutions in biotechnology. Contact me at kmohanty@student.unimelb.edu.au or +61 481 868 607.
Cell Culture
Development of a Novel oral vaccine with Vaxmed: Project involved the transformation of a liquid vaccine formulation into a solid form, namely a tablet. Experience gained in research and formulation development of a novel oral vaccine using synVAX technology, aimed at improving vaccine distribution in regions with limited healthcare infrastructure. Key components for the vaccine, including bulking agents, enteric coatings, and freeze-drying methods. Comparative analysis of oral vs. injectable vaccines, highlighting logistical advantages such as ease of administration and storage stability. A multi-disciplinary team to present a comprehensive report on manufacturing feasibility and regulatory compliance for oral vaccines. The delivery of this project helps set up the foundation of knowledge for testing formulation that can help make an oral vaccine using the synVAX technology. This would allow the company to conduct further research and trails to test out the effectiveness and efficacy of the oral vaccine using the findings of our project.
Green Synthesis of Silver Nanoparticles: Utilized lemon pulp to synthesize silver and copper nanoparticles, characterized using UV-Vis spectroscopy, FTIR, Zeta potential, and size analysis. Assessed antibacterial activity through agar diffusion, and anti-diabetic activity via inhibition assays and glucose diffusion studies. These nanoparticles demonstrated notable inhibition of alpha-amylase and glucose diffusion as well as anti-bacterial activity.
Production of Nitrogen-Rich Manure: Designed manure to replace chemical fertilizers, involving soil collection, Azotobacter medium preparation, serial dilution, sub-culturing, and nitrogen determination using the Kjeldahl method. Developed nitrogen-rich manure, achieving a 25% increase in nitrogen content. Soil studies showed improved fertility with higher microbial activity.
Antibacterial Properties of Honey: Examined honey’s antibacterial effects on Staphylococcus aureus, Proteus vulgaris, and Escherichia coli using agar diffusion. Honey exhibited strong antibacterial activity, especially against Staphylococcus aureus, showing clear inhibition zones in the agar well diffusion assay.