Loshanan Sivapalan

• Supervise the routine production and R&D activities of the various viral vaccine projects.
• Lead lab sessions to maintain and subculture cell stocks, virus infection, and antigen harvesting.
• Monitor in-process quality testing of viral vaccine, including virus titration, virus inactivation testing, sterility testing of antigen and media batches, etc.
• Calculation of virus titer for antigen batches and vaccine batches
• Testing FBS batches to meet cells and virus production standards.
• Supervise batch filling and QC testing of vaccine batches.
• Formulation of vaccine batches in consultation with lab manager.
• Maintenance and cryopreservation of cells and virus seeds stock
• Assessing personal and environmental monitoring records of lab sessions.
• Ensure that all records are appropriately maintained.
• Provide training and guidance to team members.
• Assist in drafting SOPs and preparation of forms for virology lab.
• Maintain lab supply inventory, antigen production records, and media usage records.
• Liaise with quality team to maintain calibration and validation records of equipment and labs.
• Assist in APVMA, OGTR, and DAWE audits.
• Tracking all vaccine batch records and related documents per GMP guidelines.
• Facilitate continuous improvements by ensuring long-term corrective actions are incorporated into process to prevent reoccurrence.

Key Achievements:- Independently worked on new equipment to figure out normal operations and trained fellow employees.

• Participate in the manufacturing and R&D activities of the various viral vaccine projects according to set standards.
• Maintenance and subculture of cell stocks in flasks, roller bottles, and multi-well plates.
• Perform various steps of antigen production: virus infection, freeze-thaw of roller bottles, harvesting, centrifugation, etc.
• Preparation of cell culture media batches by sterile filtration
• Perform in-process quality testing of viral vaccines, including virus titration, virus inactivation testing, sterility testing of antigen and media batches, etc.
• Assist in environmental and personal monitoring during lab sessions and liaise with quality team to manage availability of plates and keep records.
• Weekly/Monthly stock take of laboratory consumables and chemicals.
• Emulsification and filling of vaccine batches in cleanroom facility.
• Quality control testing of emulsified product: droplet test, microscopic examination, and gravity test).
• Keeping records on assigned forms as per GMP compliance
• Assist in APVMA, OGTR, and DAWE audits.
• Facilitate continuous improvements by ensuring long-term corrective actions are incorporated into process to prevent reoccurrence.

Key Achievements:- One of the pioneers and a key member of the Virus Transport Medium(VTM) Project at Treidlia Biovet during Covid(June 2020- Dec 2021).

• Preparation of materials: washing, packing, and labelling of centrifuge bottles, tubing, and media tanks for autoclaving.
• Daily monitoring of recommended temperatures of virology facilities: labs, cool rooms, refrigerators, freezers, and incubators.
• Replenish consumables and chemicals in virology lab.
• Routine cleaning, organization, and fumigation/disinfection of virology lab.
• Planned cleaning of equipment: roller racks, refrigerators, freezers, CO2 incubator, centrifuges, etc.
• Logistic support for transferring materials: media tanks, adjuvant drums, roller bottle boxes, filled vaccine tubs, etc.
• Daily disposal of quarantine, clinical, and general lab waste into assigned bins.
• Assist in maintenance and subculture of cell stocks in flasks, roller bottles, and multi-well plates.
• Assist in various steps of antigen preparation: virus infection, freeze-thaw of roller bottles, harvesting, centrifugation, etc.
• Assist in preparation of cell culture media batches by sterile filtration.
• Assist in emulsification and filling of vaccine batches in cleanroom facility.
• Keeping records on assigned forms as per GMP compliance.

• Rejuvenation of bacterial cultures and cell lines from various seed cultures.
• Propagation of starter and intermediate bacterial cultures using rejuvenated strains.
• Sampling of cultures for Quality control(QC) testing and examining and studying the bacterial growth.
• Enumeration of cultures.
• Various microbiological streaking methods for rejuvenation, purity checks and bacterial growth.
• Maintaining aseptic conditions in laboratories and clean room by following Good Laboratory Practices.
• Maintaining and monitoring all consumables and equipment used in laboratory to ensure compliance to sterility standards.

• Assisted in research and development of leaching process of final product.

Key Achievement:- Contributed to the improvement of the manufacturing process.

• Assisted in analysis of chemical and biological samples.
• Prepared reagents and solutions for experiments.