Experience in various Microbiological and Molecular Biology, Biochemistry and Immunology techniques. Proven experience working in cutting-edge research environments in various research groups.
Working in a production department in a Food safety and Compliance team. Assisting Food Safety and Compliance Coordinator in the Hazard Analysis Critical Control Point (HACCP) system.
Involved in the shotgun metagenomics project to study the interactions of microbe and host in pancreatic and colon cancer and in metastasis.
Performed DNA Extraction, Gel Electrophoresis, PCR, sample preparation for NGS.
Assisted in screening people entering the hospital to avoid the spread of COVID-19. Performed risk assessment questionnaires for visitors and patients, data entry, and communication with the medical staff.
Contributed to the project 'The interaction of PKA and CDK signalling'.
Performed DNA extraction, Gel electrophoresis, PCR, Fluorescence microscopy and SDS-PAGE Western blotting.
Contributed to the project 'Editing mutation present in the human fibroblast cell line PEX1-G843D using RESTORE and ASOs.'
Performed DNA/RNA extraction, PCR, immunofluorescence staining and Fluorescence microscopy.
Analyzed microbial and physio-chemical parameters of the water samples using the kit method. Performed sample collection, on-site sample analysis, and data entry.
Collected and analyzed the poultry samples to screen for different antibiotic residues using the disc assay method.
Playing Chess and Football, Literature, Hiking.
A Comparative Study on Dye Degradation by Leaf and Root Extracts of Parthenium hysterophorus L
(D. Shahi and R. Sapkota (2018) Int. J. Appl. Sci. Biotechnol. Vol 6(4): 327-331)
Screening of antibiotic residue in poultry in Kathmandu valley of Nepal: a cross-sectional study
(Sapkota R, Raut R, Khanal S, Gyawali M, Sahi D. Arch Pharm Pharma Sci. 2019; 3: 079-081)