Shiva Muthuswamy
Brisbane,QLD
Summary
Dynamic research professional with hands-on experience and specialization in optimizing downstream purification processes. Proficient in advanced chromatography techniques and skilled in data interpretation, I consistently enhance yield and efficiency. Adept at collaborating in cGMP environments, I drive continuous improvement initiatives while maintaining rigorous documentation standards.
Overview
2
2
years of professional experience
Work History
Junior Research Associate
Theragen Molecular Innovation Pvt. Ltd.
Chennai, India
02.2021 - 06.2021
- Optimized downstream purification techniques and conditions of IgY antibodies using affinity and hydrophobic interaction chromatography, improving purity and recovery rates.
- Performed quantitative and qualitative analysis (Bradford and Western blot) to monitor yield and assess unit operation efficiency across the purification process.
- Optimized conditions for scaling up from small-scale to pilot-scale batches, with improved consistency, yield, and throughput.
Research Executive
Saksin Lifesciences Pvt. Ltd.
Chennai, India
04.2019 - 02.2021
- Planning and execution of downstream purification activities to meet project timelines.
- Performed multistep, end-to-end unit operations for pilot-scale purification of recombinant antibody fragments, ensuring process robustness and reproducibility.
- Hands-on experience with multiple chromatographic techniques, including affinity, ion-exchange, and size-exclusion chromatography, as well as tangential flow filtration for pilot-scale batches (30 L).
- Performed yield quantification and process monitoring ,establishing efficiency benchmarks across unit operations using validated analytical methods.
- Worked within a cGMP-compliant environment, with exposure to QA/QC procedures, and regulatory documents.
- Interpreted analytical and process data, contributing to process optimization efforts and batch release data.
- Maintained documentation in line with GLP and cGMP standards, including batch records and process logs.
- Authored and implemented SOPs, supported training, standardization, and continuous improvement initiatives.
Education
Higher Degree By Research -
University of Queensland
Brisbane, QLD Masters in Molecular Medicine (M.Tech) -
Amrita Vishwa Vidyapeetham
Kochi, India01.2018
Bachelor of Technology in Industrial Biotechnology (B.Tech) -
SASTRA UNIVERSITY
India06-2014
Skills
- Molecular biology techniques: DNA & RNA extraction, electrophoresis (agarose, SDS-PAGE, and native PAGE), RT-PCR reaction, Western blot, silver staining, and protein estimation techniques
- Protein purification & downstream techniques: Hands on experience with affinity, IEX, SEC and HIC chromatography, skilled in column packing and process optimization
- Instrumentation Expertise: Proficient in operating AKTA systems (Prime, Pure & Go), high pressure homogenizer, pilot scale and lab-scale TFF systems, FT-IR & Rheometer
- Mammalian cell and microbial techniques: Experienced in mammalian cell culture, cell based functional assays, standard microbial techniques and confocal microscopy imaging and analysis
- Proteomics and bioinformatics: familiar with sample processing for proteomics, pathway enrichment analysis, and protein-protein interaction network analysis using bioinformatics tools
Research Experience
1. Ph.D. Thesis (October 2021 - Present), Title: Biophysical cues regulating osteocytes: Implications for Bone remodelling
- Developed and characterized osteocyte 3D model system by functionalizing synthetic hydrogel.
- Designed and performed cell-based assay protocols to assess functional responses of osteocytes.
- Performed confocal imaging & quantitative analysis of osteocyte morphology and dendritic architecture.
- Prepared samples for label-free quantitative proteomics, including cell lysis, protein digestion and desalting for LC-MS/MS.
- Performed bioinformatic analysis of proteomics data, including GO/KEGG enrichment, protein-protein interaction networks and interpretation.
2. Master's Dessertation (June 2017 – July 2018), Title: Anti-staphylococcal and Neutrophilic Chemotactic Injectable κ-Carrageenan Hydrogel for Infectious Wound Healing.
- Developed an injectable κ-carrageenan based hydrogel ad characterized its physiochemical, drug release kinetics and rheological properties.
- Isolated neutrophils from human blood and performed cell based assays to evaluate biocompatibility and immunomodulatory properties of hydrogel.
- Handled Sprague Dawley rat model to assess invivo antibacterial efficacy of hydrogel.
3. Bachelor's Thesis (Jan 2013 – May 2014), Title: Inhibiting biofilm formation of S.aureus in CAUTI through quorum sensing.
- Determined minimum inhibitory concentration (MIC) of inhibitor(AIP2) to inhibit biofilm formation.
- Evaluated the anti-biofilm efficacy of inhibitor (AIP2) in clinical strains of S.aureus of catheter associated urinary tract infection (CAUTI) model.
Accomplishments
- Awarded People's Choice Award for Intra-School three minute thesis (3MT) competition.
- Qualified at the national level in GATE 2015 (Graduate Aptitude Test in Engineering) in the biotechnology stream.
- Qualified as Trainee in the Biotechnology and Biopharmaceutical Skill Enhancement Program (B2SEP), receiving a scholarship for hands-on training in biopharma manufacturing techniques.
Publications
- Taylor, M. A., Choi, J. U. A., Muthuswamy, S., Martinez, M. A. E., Lauko, J., Kijas, A. W., & Rowan, A. E. Sensitive label free imaging of 3D cell models with minimal toxicity using confocal reflectance. Biomaterials science.2024 (I.F-5.7).
- Shiva M, Viswanathan A, Yegappan R, Selvaprithiviraj V, Vasudevan AK, Biswas R, Jayakumar R. Antistaphylococcal and neutrophil chemotactic injectable kcarrageenan hydrogel for infectious wound healing. ACS Applied Bio Materials. 2018. (IF:4.7)
- Aparna V, Shiva M, Biswas R, Jayakumar R. Biological macromolecules based targeted nanodrug delivery systems for the treatment of intracellular infections. International journal of biological macromolecules. 2018. ( IF:4.7 )
References
References available upon request.
Timeline
Junior Research Associate
Theragen Molecular Innovation Pvt. Ltd.
02.2021 - 06.2021
Research Executive
Saksin Lifesciences Pvt. Ltd.
04.2019 - 02.2021
Higher Degree By Research -
University of Queensland
Masters in Molecular Medicine (M.Tech) -
Amrita Vishwa Vidyapeetham
Bachelor of Technology in Industrial Biotechnology (B.Tech) -
SASTRA UNIVERSITY
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