Tanisha Sharma
Salisbury,Qld
Summary
A fresh graduate with a Master of Pharmaceutical Industry Practice from the University of Queensland and a solid foundation in Biomedical Science. A year of practical experience as an mRNA Research Intern at BASE mRNA Facility, where I honed my research skills and contributed to groundbreaking projects. Passionate about advancing pharmaceutical research through innovative methodologies and dedicated teamwork.
Overview
3
3
years of professional experience
1
1
Certification
Work History
Research Intern (Mercer Lab)
Base mRNA Facility | Australian Institute of Bioengineering and Nanotechnology, University of Queensland
06.2023 - 06.2024
- Conducted library preparation from 9 g-Block PCR products using the Rapid Barcoding Kit (RBK114.24) to compare the error rates with plasmid constructs for in vitro transcription of mRNA
- Contributing in Developing a script for poly(A) tail analysis of plasmid and g-Block constructs, contributing to enhanced RNA stability and quality assessment
- Utilized ONT Direct-cDNA sequencing to prepare libraries from eGFP plasmid, conducting comprehensive analyses including poly(A) tail length, error rates, and primary read lengths, crucial for optimizing mRNA vaccine formulations
- Analyzed error rates and poly(A) tail characteristics of HA and MLI g-Block libraries prepared using the ONT ligation sequencing kit (NBD114 Kit), further refining sequencing protocols for improved vaccine efficacy
- Optimized ONT Direct-cDNA sequencing protocols using Huh-7 Total RNA cells transfected with eGFP, establishing methods for quantifying mRNA integrity post-transfection and developing an anchored primer for poly(A) tail length analysis
- Applied Bioinformatics Analytical pipeline to perform the analyses as well as tools such as snapgene and IGV.
Pharma research intern
Society for Health and Medical Technology | India
08.2021 - 01.2023
- Developed a database for symptom and disease-based diagnosis support
Education
Master of pharmaceutical industry practice, Drug Design and Formulation, Pre-clinical drug Development, Clinical drug development, biometrics -
University of Queensland
07.2024
BSc IN BIOMEDICAL SCIENCES, Biochemistry, Clinical Chemistry, Immunology, Pharmacology, Hematology, Medical Microbiology, Molecular Genetics, Practical skills in biomedical science, Cell and system biology, statistics for Life Sciences -
La Trobe University
02.2022
Skills
- Molecular Biology techniques such as PCR, ELISA, cell culture, DNA/RNA Extraction, Western Blot bacterial cloning, protein purification and etc
- ONT sequencing
- Communicating Biological research findings using scientific writing
- Careful data handling and analysis
- NCBI Blast
- Clustal omega
- Interpro
- SPSS
- UniProt
- PSORT
- ExPASy
Accomplishments
· Good Clinical Practice by NIDA Clinical Trials Network
· Bacterial Genomes I: From DNA to Protein function using Bioinformatics by Wellcome Connecting Science
· Bacterial Genomes III: Comparative Genomics Using Artemis Comparison Tool(ACT) by Wellcome Connecting Science
Coursework
- Good Clinical Practice by NIDA Clinical Trials Network
- Bacterial Genomes I: From DNA to Protein function using Bioinformatics by Wellcome Connecting Science
- Bacterial Genomes III: Comparative Genomics Using Artemis Comparison Tool(ACT) by Wellcome Connecting Science
Projects
- Genome annotation using a series of bioinformatics tools Research project – “How safe and efficient are mRNA vaccines?”. I focused on the safety and immunogenicity of mRNA vaccines in comparison to conventional vaccines for three different diseases- Zika, Rabies and Covid-19.
- Scientific News and Views Article- “Caspase-9 cleavage managed by Caspase-3 leads to full activation of the apoptosome.
- Conducted Lab experiments and communicated my research findings in a scientific report. Two of them include- “Immobilized metal affinity chromatography (IMAC) to purify Hexahistidine tagged GFP protein from transformed E.coli XL1 cells.” And “isolation and purification of mitochondria from the meristem of a cauliflower”.
- Research poster- Explored potential in vivo and in vitro experiments for a fictional drug with apoptotic properties similar to caspase-3, demonstrating its potential for decreased tumor growth in pancreatic cancer.
Certification
· Good Clinical Practice by NIDA Clinical Trials Network
· Bacterial Genomes I: From DNA to Protein function using Bioinformatics by Wellcome Connecting Science
· Bacterial Genomes III: Comparative Genomics Using Artemis Comparison Tool(ACT) by Wellcome Connecting Science
Timeline
Research Intern (Mercer Lab)
Base mRNA Facility | Australian Institute of Bioengineering and Nanotechnology, University of Queensland
06.2023 - 06.2024
Pharma research intern
Society for Health and Medical Technology | India
08.2021 - 01.2023
Master of pharmaceutical industry practice, Drug Design and Formulation, Pre-clinical drug Development, Clinical drug development, biometrics -
University of Queensland
BSc IN BIOMEDICAL SCIENCES, Biochemistry, Clinical Chemistry, Immunology, Pharmacology, Hematology, Medical Microbiology, Molecular Genetics, Practical skills in biomedical science, Cell and system biology, statistics for Life Sciences -
La Trobe University
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