Tharun Adapa

Working as a Research assistant in Global Centre for Gynaecological Diseases at University of Newcastle. My expertise here is cell culture, histology, 2D and 3D Imaging, Image analysis and quantification to study cancer growth and origin targeted to female reproductive tract such as Ovarian cancer, Endometrial cancer and gynaecological diseases like Endometriosis.

• Histology Experiments like Immunofluorescence, Immunohistochemistry, H&E, In Vitro hybridization, Tunnel assay, Nuclear fast Red staining etc.
• ·Microscopy techniques like Confocal and Slide scanner.
• Computation and Image analysis by using software tools like Q-path, HALO, Image-J, Olyvia, and Arivis.
• ·Experienced in fixing samples in PFA or formalin, Paraffin tissue sectioning, Paraffin Block making, 3D immunofluorescence staining by using different clearing processes like CUBIC-R+.

Testing the battery performance of the batteries with new materials to prepare a sustainable battery at a low cost. Testing the conductivity of some biopolymers that are used in drug delivery. This research project is supervised by Professor Prashant Sonar, Dr. Jun Mei, and Dr. Amandeep Singh Pannu.

• Slurry preparation for battery by blending the Biopolymers with conduction polymers.
• Performed characterization techniques like Atomic Force Microscopy, Scanning Electron Microscope, and X-ray Photoelectron Spectroscopy to know the physical-mechanical and chemical details of thin films.
• This film is prepared and punched into cells for the battery assembly.
• Studying the performance of the Bio-composite battery through battery testing procedures.

Cloning and Bead production by using E. coli for the desired protein that binds to Silica which can be used for the Drug delivering purpose in Anti-cancer drugs for Colon cancer. The desired proteins are expressed through SDS – the PAGE technique, which is further useful for Drug Delivery Research. This research project is supervised by Professor Bernd Rehm and Dr. David Wibowo.

• Performed cloning techniques like Plasmid Isolation, Restriction Digestion, Agarose gel Electrophoresis, DNA Gel extraction, Ligation, and Transformation.
• Working on the Production of Bead Isolation, purification, and sterilization.
• Working with chromatography techniques like Sodium Dodecyl Sulphate – Polyacrylamide gel electrophoresis (SDS – PAGE) and Preparation of SDS Gels.
• Handling of High-Performance Liquid Chromatography.

Studied Mfg. of pertussis bulk antigen from the strains 134 and 509 of Bordetella pertussis that is used in trivalent DPT vaccine as an Undergraduate Intern. This project is supervised by Dr. Pavan Kirpan (Manager, Biological E Ltd, Pertussis Production Department.

• Studied Mfg. of pertussis bulk antigen from the strains 134 and 509 of Bordetella pertussis that is used in the trivalent DPT vaccine.
• Handling equipment like a Fermenter, U.V. Spectrometer, Dissolve Oxygen Probe, Agitator, Alkali-Acid Supply Pump, Incubator, and Laminar Air Flow.
• Working on the Production of Bead Isolation, purification, and sterilization.