Undertaking experiments in vitro and in vivo investigating the ability of nanoparticles to deliver chemotherapy agents specifically to tumours while avoiding undesirable side effects in a clinically relevant animal cancer model
Cell culture assays
Flow cytometry
Animal studies.
Research Assistant, Grade 2
University of Melbourne
09.2008 - 07.2012
Assisting post-docs/students with their research projects
Animal surgeries/injections/monitoring/end-point analyses (muscle function testing)
Cell culture, Western blotting, qPCR, immunostaining
Maintaining mice colonies including genotyping
General lab maintenance.
Research Assistant, Grade 1
University of Melbourne
01.2003 - 01.2007
Animal experiments involving jugular vein cannulation and infusions of radioactively labelled compounds
Processing samples collected for analyses such as cutting, staining and imaging, Western blotting
Maintaining mice colonies including genotyping
General lab maintenance.
Education
Honours Degree in Science (Anatomy) -
University of New South Wales
12.2002
Bachelor of Biomedical Science (BBioMedSc) -
University of Melbourne
12.2001
Skills
Teamwork and Collaboration
Microsoft Office
Time Management
Problem-Solving
Data Collection
Multitasking and Organization
Data Entry
Analytical Thinking
Data Analysis
Documentation skills
Project Management
Laboratory Operations
Scientific Experiments
Lab Equipment Operation
Experimental design
Project Planning
Skillsandresponsibilities
Proficient in molecular biology techniques such as SDS-PAGE electrophoresis, Western immunoblotting, DNA/RNA/protein extraction, restriction enzyme digests, plasmid preps.
Extensive experience in animal surgery and dissections (in rats and mice) - including anesthesia, oral gavage, intravenous injections, intraperitoneal injections, skeletal muscle injections and function testing (in situ), jugular vein cannulations and infusions, spinal cord injections, inoculation of tumour cells,
Considerable experience in cell culture techniques including culturing muscle cells, cancer cells; 3D cell cultures and primary cell culture (and some experience with flow cytometry)
Proficient in performing histological techniques such as cryostat sectioning, immunofluorescent and immunoperoxidase staining of tissue sections and cells.
Experience in imaging including confocal microscopy
Managing mice breeding programs including genotyping.
Maintenance of general lab area and stocks
Maintenance of good and accurate lab records
Administrative tasks such as ordering, budgets, invoice management and account reconciliations
Personal Qualities
Highly organized with a high level of interpersonal skills
Extensive range of lab skills with great attention to detail
Able to work unsupervised and as part of a team
Strong commitment to quality and adhering to the principles of good laboratory practices
Able to multi-task projects to meet team deadlines in a challenging, fast-paced environment
Flexible attitude including capacity to acquire new skills