Lizebona August Ambattu
Melbourne
Summary
Detail-oriented Research Assistant with hands-on experience performing clerical and laboratory work to advance research goals. Drives team success with expertise in data review, reporting and specimen handling. Specializes in [Area of study].
Overview
12
12
years of professional experience
1
1
Certification
Work History
Research Assistant
RMIT University
10.2022 - Current
- Investigating “Sonomechanobiology: Mechanotransduction induced by High-Frequency Acoustics” with focus on membrane mechanotransduction and nuclear mechanotransduction
- Contributing to development of novel high-frequency acoustics based diagnostic and therapeutic systems for cancer therapy, regenerative therapy, exosome therapy and neurodegenerative diseases
- Skills: Mammalian cell culture, 3D cell culture, cell-based assay, Microscopy, Western blotting, SDS-PAGE, RIP, RT-PCR, CHIP, ELISA, Data management and analysis, Data representation, SOP development, Image analysis
Doctoral Fellow
RMIT University
03.2019 - 10.2022
- Established the mechanotransduction induced by high-frequency acoustics in adherent mammalian cells, particularly mesenchymal stem cell, endothelial cells and cancer cells
- Identified the involvement of calcium signalling and cAMP signalling leading to transient or permanent changes in acoustically stimulated cells
- Proved the involvement of calcium store, endoplasmic reticulum, in acoustically excited cells
- Independently screened and developed protocol for manipulating cells using high-frequency acoustics to improve cellular activities like exosome generation, stem cell differentiation and endothelial barrier integrity
- Analyzed and presented data to publish 4 first-author publications, a second-author publication and 4 Australian provisional patent applications (2022903491, 2022903307, 2022903305, 2020902838)
- Key Projects: Mechanobiology
- High-frequency acoustic cell stimulation promotes exosome generation regulated by a calcium-dependent mechanism
- Short-Duration High-Frequency MegaHertz-Order Nanomechanostimulation Drives Early and Persistent Osteogenic Differentiation in Mesenchymal Stem Cells
- Calcium-dependent cAMP mediates the mechanoresponsive behaviour of endothelial cells to high-frequency nanomechanostimulation
Senior Research Associate
SciGenom labs
12.2015 - 04.2018
- Developed, validated, and standardized analytical methods and ensured accurate and reliable data acquisition while leading the main R&D project for the establishment of reagent-grade monoclonal antibodies using CHO, HEK293 and Expi293 and assisting the accessory projects for the development of ELISA-based assay development and magnetic bead for protein isolation (XpressAffinity-Protein Beads)
- Isolated and characterised reagent-grade monoclonal antibodies using ELISA, immunofluorescence, flow cytometry and Western blotting
- Troubleshot problems while multi-tasking using problem-solving and time management skills in molecular cloning, resulting in development of protocols that increased efficiency by 40%
- Prepared reports for product development and validation and developed SOPs to isolate and screen monoclonal antibodies from the phage display library
- Skills: Mammalian cell culture, Phage display library propagation, maintenance and storage, Phage display library screening and isolation, protein isolation, purification and characterisation, molecular cloning, cell-based assay, Microscopy, Western blotting, SDS-PAGE, ELISA, Data management and analysis, Data representation, SOP development, Image analysis
Junior Research Fellow
SCTIMST
02.2013 - 08.2015
- Developed and characterized polymer-based nucleic acid delivery systems for developing 6 nanocarriers targeting the blood-brain barrier
- Identified modifiers and designed delivery systems that target blood brain barrier leading to 2 papers in peer-reviewed journals and 1 review paper
- Skills: Mammalian cell culture, Microscopy, ELISA, Nanoparticle design and characterisation, Cell-based assay, Small animal handling, Animal model (tumor) development, Subcutaneous injection, Tail veil injection, Dissection and tissue collection for processing, Data management and analysis, Data representation
Education
Ph.D. - Mechanobiology
RMIT University
Melbourne, VIC05-2023
Skills
- Teamwork and collaboration
- Microsoft office
- Time management
- Problem-solving
Accomplishments
- Presented research on [Topic] at [Conference Name].
- Made breakthrough in [Area of research] in [Year].
- Delivered poster presentation on [Topic] at [Conference Name] in [Year].
- Collaborated with investigators at [Institution Name] to make breakthrough in [Area of research].
Certification
- [Area of certification] Training - [Timeframe]
- Certified [Job Title], [Company Name] - [Timeframe]
- [Area of certification], [Company Name] - [Timeframe]
- [Area of expertise] License - [Timeframe]
- Licensed [Job Title] - [Timeframe]
Timeline
Research Assistant
RMIT University
10.2022 - Current
Doctoral Fellow
RMIT University
03.2019 - 10.2022
Senior Research Associate
SciGenom labs
12.2015 - 04.2018
Junior Research Fellow
SCTIMST
02.2013 - 08.2015
- [Area of certification] Training - [Timeframe]
- Certified [Job Title], [Company Name] - [Timeframe]
- [Area of certification], [Company Name] - [Timeframe]
- [Area of expertise] License - [Timeframe]
- Licensed [Job Title] - [Timeframe]
Ph.D. - Mechanobiology
RMIT University
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