Professional microbiologist with deep knowledge in laboratory procedures and keen eye for detail. Adept at collaborating within teams and adaptable to changing environments. Skilled in experimental design, data analysis, and safety protocol adherence. Known for reliability and delivering impactful results.
1. Screening and molecular characterization of potential phosphate solubilizing bacteria to be used as Phosphorus biofertilizer from root-associated soil of rice plants of different regions in Bangladesh. (Role: PI) (Funded by Research Cell, NSTU)
2. Molecular detection of virulence genes, plasmid profiling, and antibiotic susceptibility of Salmonella spp. isolated from faecal sludge of different regions in Bangladesh. (Role: PI) (Funded by Research Cell, NSTU).
3. Multiple drug-resistant bacteria emerged from untreated hospital associated wastewater: a potential threat to public health in southeastern region of Bangladesh. (Role: PI) (Funded by Ministry of Science and Technology)
4. Prevalence, antibiotic resistance pattern and genes of uropathogens isolated from unconcerned university going young women with Urinary tract infection (UTI) in Noakhali, Bangladesh. (Role: PI) (Funded by Ministry of Science and Technology)
5. An empirical intervention of a single training on aseptic techniques to lab personnel in a month and fortnightly fumigation of rural community microbiology laboratory: impacts on reducing blood culture contamination (Role: Co-PI, Funded by Bangladesh Govt.)
1. Pneumococcal Conjugate Vaccine (PCV) impact study in Bangladesh. (Funded by BMGF)
2. Assessment of effectiveness of Pneumococcal Conjugate Vaccine-10 on Nasopharyngeal Carriage in Bangladesh. (Funded by BMGF & GSK).
3. Collection of Nasopharyngeal Swabs from Patients with Severe ALRI for Molecular Detection of Respiratory Viruses. (Funded by BMGF)
4. To measure the immunogenicity of PCV-10 and to link serotype specific antibody achieved after 3 doses to the impact of PCV-10 on serotypes in lab isolates. (Funded by BMGF)
5. Screening and Treatment of maternal genitourinary infections in early pregnancy to prevent preterm birth in rural Sylhet, Bangladesh: a cluster randomized trial. (Funded by NIH)
Basic Microbiology
Pathogen isolation from clinical specimens (blood, CSF, urine, pus) • Antibiotic susceptibility testing • Staining techniques (Gram, acid-fast, simple) • Media preparation • Sterilization methods (dry, wet) • Sterility testing • Antibiotic assays • Coliform quantification (MPN) • Blood culture systems (conventional, automated)
Molecular Biology
DNA extraction (conventional and kit-based methods) • Plasmid isolation • Agarose gel electrophoresis • PCR techniques (conventional, real-time, multiplex) • Flow cytometry for cell cycle analysis • Sonication for cell disruption and DNA/protein extraction • Molecular cloning (restriction digestion, ligation, transformation)
Immunology
Latex agglutination • ELISA (antigen/antibody detection) • Multiplex detection (Luminex Bioplex) • Western blotting
Biochemistry & Proteomics
Enzyme assays (spectrophotometric) • Protein quantitation (Bradford, Lowry, BCA) • Protein separation (SDS-PAGE) • Chromatography (paper, TLC, LC-MS) • Serum separation • Agglutination assays • Serotyping
Cell Biology
Human and animal tissue culture techniques • Cytotoxicity assays • Cell proliferation assays • Co-culture assays
Bioinformatics
Protein characterization • Vaccine design • Immunostimulation analysis • Molecular docking • Molecular dynamic simulation
Animal Handling
Laboratory animal handling • Animal injection techniques • Animal sample collection • Animal monitoring
Data Analysis
MS Excel • GraphPad Prism • Statistical analysis • Data visualization
Laboratory Management
Laboratory inventory management • Laboratory housekeeping • Risk assessment preparation • Safety protocol implementation
Research Administration
Writing human and animal ethics applications • Grant writing • Research project planning • Scientific documentation
Research Articles
15. Rahman, M. M., Masum, M. H. U., Parvin, R., Das, S. C., & Talukder, A. (2025). Designing of an mRNA vaccine against high-risk human papillomavirus targeting the E6 and E7 oncoproteins exploiting immunoinformatics and dynamic simulation. PloS one, 20(1), e0313559. https://doi.org/10.1371/journal.pone.0313559
14. Masum, M. H. U., Wajed, S., Hossain, M. I., Moumi, N. R., Talukder, A., & Rahman, M. M*. (2024). An mRNA vaccine for pancreatic cancer designed by applying in silico immunoinformatics and reverse vaccinology approaches. Plos one, 19(7), e0305413. https://doi.org/10.1371/journal.pone.0305413
13. Rahman, M. M*., Grice, I. D., Ulett, G. C., & Wei, M. Q. (2024). Advances in Bacterial Lysate Immunotherapy for Infectious Diseases and Cancer. Journal of Immunology Research, 2024(1), 4312908. https://doi.org/10.1155/2024/4312908
12. Rahman, M. M*., Masum, M. H. U., Talukder, A., & Akter, R. (2023). An in silico reverse vaccinology approach to design a novel multiepitope peptide vaccine for non-small cell lung cancers. Informatics in Medicine Unlocked, 37, 101169. https://doi.org/10.1016/j.imu.2023.101169
11. Talukder, A., Rahman, M. M.*, & Masum, M. H. U. (2022). Biocomputational characterisation of MBO_200107 protein of Mycobacterium tuberculosis variant caprae: a molecular docking and simulation study. Journal of Biomolecular Structure and Dynamics, 1-20.
10. Baten, N., Wajed, S., Talukder, A., Masum, M. H. U. & Rahman, M. M.* (2022) Coinfection of fungi with SARS-CoV-2 is a detrimental health risk for COVID-19 patients. Beni-Suef Univ J Basic Appl Sci 11, 64. https://doi.org/10.1186/s43088-022-00245-9
9. Rahman, M. M., Masum, M. H. U., Wajed, S., & Talukder, A. (2022) A comprehensive review on COVID-19 vaccines: development, effectiveness, adverse effects, distribution and challenges. VirusDisease, 1. https://doi.org/10.1007/s13337-022-00755-1
8. Amin, S., Aktar, S., Rahman, M. M., & Chowdhury, M. M. H. (2022). NLRP3 inflammasome activation in COVID-19: an interlink between risk factors and disease severity. Microbes and Infection, 24(1): 1-10. https://doi.org/10.1016/j.micinf.2021.104913
7. Uddin, M. S., Rahman, M. M.*, Faruk, M. O., Talukder, A., Hoq, M. I., Das, S., & Islam, K. M. (2021). Bacterial gastroenteritis in children below five years of age: a cross-sectional study focused on etiology and drug resistance of Escherichia coli O157, Salmonella spp., and Shigella spp. Bulletin of the National Research Centre, 45(1), 1-7. https://doi.org/10.1186/s42269-021-00597-9
6. Talukder, A., Rahman, M. M.*, Chowdhury, M. M. H., Mobashshera, T. A., & Islam, N. N. (2021). Plasmid profiling of multiple antibiotic-resistant Pseudomonas aeruginosa isolated from soil of the industrial area in Chittagong, Bangladesh. Beni-Suef University Journal of Basic and Applied Sciences, 10(1), 1-7. https://doi.org/10.1186/s43088-021-00131-w
5. Rahman, M. M., Talukder, A., & Akter, R. (2021). Computational Designing and Prediction of ADMET Properties of Four Novel Imidazole-Based Drug Candidates Inhibiting Heme Oxygenase-1 Causing Cancers. Molecular informatics. https://doi.org/10.1002/minf.202060033
4. Rahman, M. M.*, Devnath, P., Jahan, R., & Talukder, A. (2021). Detection of multiple antibiotic-resistant bacteria from the hospital and non-hospital wastewater sources of a small town in Noakhali, Bangladesh. Journal of Applied Biology & Biotechnology Vol, 9(3), 59-65. https://doi.org/10.7324/JABB.2021.9308
3. Rahman, M. M.*, Talukder, A., Chowdhury, M. M. H., Talukder, R., & Akter, R. (2021). Coronaviruses in wild birds–A potential and suitable vector for global distribution. Veterinary Medicine and Science, 7(1), 264-272. https://doi.org/10.1002/vms3.360
2. Rahman, M. M.*, Ferdouse, J., Akter, R., Uddin, M. S., Aktar, S., Paul, S. C., ... & Anwar, M. N. (2019). In vitro evaluation of probiotic and bacteriocinogenic potentiality of Lactobacillus plantarum and Lactobacillus delbrueckii isolated from vegetables in Chittagong region, Bangladesh. Malaysian Journal of Microbiology, 15(2), 132-142. http://dx.doi.org/10.21161/mjm.180070
1. Rahman, M. M.*, Aktar, S., Faruk, M. O., Uddin, M. S., Ferdouse, J., & Anwar, N. (2018). Probiotic Potentiality of Lactobacillus coryniformis subsp. Torquens MTil and Lactobacillus coryniformis MTi2 Isolated from Intestine of Nile Tilapia: An In vitro Evaluation. Journal of Pure and Applied Microbiology, 12(3), 1037-1046. https://doi.org/10.22207/JPAM.12.3.01
International Conference Proceedings
1. Rahman, M. M., Ahmed, Z. B., Tanmoy, A. M., Islam, M., Baqui, A. H. & Saha, S. K. (2016). Too many specimens, too few positives: re-evaluation of incubation period for automated blood culture systems. Abstract accepted and won travel grant award in young scientist category, and presented as poster in 10th International Symposium on Pneumococci and Pneumococcal Diseases (ISPPD-10) held in Glasgow, UK. https://isppd2016.kenes.com/symposium-information/travel-grant-winners#.W8IcodczbIU