Nawras Abbud

Experienced scientist with a strong background in diagnostic microbiology and immunology, having worked across both public and private sectors in Australia. Skilled in microbiological diagnostics and molecular techniques, with hands-on experience in studying immune system responses and exploring molecular mechanisms underlying disease. Adept at applying scientific knowledge to diagnostic and research settings, with a commitment to advancing healthcare through innovative solutions.

• NATTI CCL (Community Language Credential) Certificate, Arabic-English CCL, 2020
• Certificate III Pathology Collection, HLT37215, In Progress

• Medical Laboratory Scientist, Australian Institute of Medical and Clinical Scientists (AIMS), 2021
• Medical Laboratory Technician, Australian Institute of Medical and Clinical Scientists (AIMS), Australia
• Medical Laboratory Scientist, The Israeli Ministry of Health, 2017

• Professional Member MAIMS, Australian Institute of Medical and Clinical Science (AIMS)
• Associate Member, Australian Society for Microbiology (ASM)
• Member, Australasian Cytometry Society (ACS)
• Member, Australian Atherosclerosis Society (AAS)

Cellular and Molecular Biology Techniques

• Proficient in SDS-PAGE and Coomassie blue staining for protein separation and visualization.
• Experienced in cell counting and viability assessment using a Hemacytometer.
• Skilled in preparing single-cell suspensions from primary tissues for downstream applications.
• Competent in performing Bradford protein assays for protein concentration measurement.
• Skilled in employing multiple DNA extraction kits for the isolation of high-purity genomic DNA from cell cultures.

Data Processing Software Expertise

• Flow cytometry data analysis using FlowJo.
• Gen5 Micro Plate Reader & Imager software for high-throughput assays.
• Experienced in GraphPad Prism for statistical analysis and data visualization.
• Skilled in Geneious for sequence alignment, primer design, and NGS data analysis.

Cell Separation and Sorting Techniques

• Expertise in immunomagnetic cell separation (MACS) for positive and negative cell selection.
• Experienced with sucrose density gradient ultracentrifugation for membrane fraction separation.

Laboratory Animal Handling

• Skilled in mouse handling, intraperitoneal injections, and survival surgery.
• Proficient in isolating mouse spleen, lymph nodes, and peritoneal cavity cells.

Flow Cytometry and Molecular Techniques

• Specialized training in flow cytometry at the Doherty Institute, with expertise in instrument operation and data analysis.
• Experienced in site-directed mutagenesis for introducing specific mutations into DNA for protein function studies.

Cell Culture and Assay Techniques

• Experienced in mammalian cell culture, including maintenance, transfection, and growth of cell lines (e.g., HEK293, 721.21).
• Skilled in performing luciferase assays to measure gene expression, promoter activity, and cellular response to treatments.

Journal:

Official Journal of The International Association of Inflammation Societies &the European Histamine Research Society 2015

Satiated macrophages up-regulate CD24 Expression through uncoupling protein 2

Saadi J., Satyanarayanan K.S., Nawras Abboud, Zuck S., Ariel A.

Presentations:

1. Research Presentation:

Faculty of Natural Sciences “the Honor's Research Program talks”, University of Haifa, November 22, 2015- Investigating macrophage phenotypes during the termination of the inflammatory response.

2. Research Presentation:

Faculty of Natural Science Posters Day, University of Haifa, June 15, 2016 - present Regulation of CD24 Expression on Resolution-Phase Macrophages Following Interferon-β Treatment

• Master of Biomedical Science, University of Melbourne,Research Subject: Investigating lineage, I (D0-D2) KIR and Natural Killer cell function

• Research Honor's Program, University of Haifa,2014, 2016, Research Subject: Investigating macrophage phenotypes during the termination of the inflammatory response. Regulation of CD24 Expression on Resolution-Phase Macrophages Following IFN- β Treatment.

• Research Program in Science, Hagalil Research and Development Centre, Franciscan sister's secondary school, 2007, Research Subject: Preparing Trans-2-Octenal (Unsaturated Aldehydes), a substance used as a flavor ingredient.