Gouthami Midatana
Protein Biochemist
Melbourne
Summary
Highly skilled Protein Biochemist with 3 years of experience in recombinant protein expression, purification, and biochemical characterization for drug discovery and therapeutic applications. Expertise in bacterial and mammalian expression systems, large-scale protein production, and purification using AKTA (Affinity, IEX, SEC). Proficient in biophysical characterization techniques to check for protein stability and binding kinetics. Strong background in molecular biology (PCR, cloning, mutagenesis), assay development, and analytical validation (HPLC, mass spectrometry). Well-versed in GMP, GLP, and ISO13485 compliance, ensuring high-quality and reproducible workflows. Adept at experimental design, troubleshooting, SOP development, and cross-functional collaboration to drive successful IND-enabling studies in a fast-paced R&D environment.
Overview
5
5
years of professional experience
4
4
Certifications
Work History
Laboratory Technician
Dorevitch Pathology- CONTRACT
07.2024 - 01.2025
- Performed nucleic acid extraction, PCR, and biochemical assays for clinical diagnostics
- Maintained quality control by calibrating lab instruments and ensuring regulatory compliance
- Assisted in data documentation and report generation following GLP guidelines
- Assisted in sample handling, preparation, and storage to maintain sample integrity
- Conducted troubleshooting and preventive maintenance of laboratory instruments to ensure uninterrupted workflow
Laboratory Assistant
Dorevitch Pathology- PART TIME
10.2023 - 06.2024
- Managed specimen processing, data entry, and compliance documentation
- Maintained laboratory safety protocols and ensured adherence to ISO13485 and TGA regulations
- Supported inventory management and reagent preparation, ensuring laboratory supplies were adequately stocked
- Assisted in documentation and compliance tracking, maintaining accuracy in data recording for audits and quality control
Senior Research Scientist
Aragen Life Sciences
08.2021 - 04.2022
- Well-versed in planning, designing, and executing assigned projects, ensuring timely delivery of data and results to clients
- Led recombinant protein expression and purification projects for diagnostic and therapeutic applications
- Designed and optimised large-scale protein production strategies using E
- Coli expression systems
- Operated AKTA chromatography systems (Avant, Pure, Start) for affinity, ion exchange, and SEC purification, including data analysis and method optimization using Unicorn software
- Conducted enzyme activity assays and biochemical characterization to validate protein integrity
- Developed ISO13485-compliant protocols for scaled-up manufacturing under GMP guidelines
- Authored technical reports, SOPs, and regulatory documentation to meet compliance standards
- Effectively collaborated with internal and external teams to manage outsourced experimental needs, troubleshoot challenges, and coordinate timely shipments with the Project Management and Logistics teams to ensure smooth project execution
- Ensured quality control at every stage of protein production, including process monitoring, assay validation, and compliance with ISO13485/GMP guidelines
Research Scientist
Aragen Life Sciences
09.2019 - 08.2021
- Expressed and purified recombinant KRAS G12D, KEAP 1, E3 ligase, and IL2/IL7 proteins for research applications
- Conducted process development and scale-up of microbial fermentation using bioreactors
- Performed analytical method validation using HPLC, UV-Vis spectroscopy, and mass spectrometry
- Performed molecular biology techniques including molecular cloning, sub-cloning, restriction digestion, ligation, agarose gel elution and cleanup, PCR, gel electrophoresis (AGE & PAGE), BCA, and western blotting
- Optimized buffer formulations for protein stability and shelf-life extension
- Ensured compliance with GMP, GLP, and ISO13485 standards in all laboratory operations
- Managed stock and inventory, maintained instrument logs and validation reports, and coordinated with vendors for timely maintenance, calibration, and addressing breakdown issues
Education
Master's in Health Administration
La Trobe University
Melbourne, VIC06-2024
Master's in Applied Microbiology
Vellore Institute of Technology
India06-2019
Bachelor's in Life Sciences
Andhra University
India04-2017
Skills
Recombinant Protein Expression
Mammalian Protein Expression( HEK293, CHO)
Chromatography ( Affinity, Ion Exchange, Size Exclusion, HPLC)
Baculovirus Expression System
Biochemical and Biophysical Characterisation(SDS-PAGE, Western Blot, Enzyme Kinetics,
Molecular Biology (PCR, Cloning, Transformation, Gel Electrophoresis)
Bacterial & Mammalian Fermentation (Shake Flask, Bioreactors, Downstream
Protein Stability & Formulation Studies (DSF, HPLC, Mass Spectrometry)
Regulatory Compliance (ISO13485, GMP , GLP , FDA & TGA Regulations)
Quality Control & Analytical Validation (Assay Development, Lot Release Testi
Project Management & Technical Documentation (SOPs, Protocol Writing, Grant proposals)
Team work and Cross-Functional Collaboration
Attention to detail and precision
Laboratory Information Management System
Certification
ISO13485 & GMP Compliance Certification
Additional Information
- Full working rights in Australia
- Flexible availability and willing to relocate
- References available upon request
Timeline
Laboratory Technician
Dorevitch Pathology- CONTRACT
07.2024 - 01.2025
Laboratory Assistant
Dorevitch Pathology- PART TIME
10.2023 - 06.2024
Senior Research Scientist
Aragen Life Sciences
08.2021 - 04.2022
Research Scientist
Aragen Life Sciences
09.2019 - 08.2021
Master's in Health Administration
La Trobe University
Master's in Applied Microbiology
Vellore Institute of Technology
Bachelor's in Life Sciences
Andhra University