Lahi Rupasinghe

Results-driven microbiology analyst with expertise in mammalian cell culture, virology assays, and molecular techniques. Proficient in DNA extraction, PCR, virus neutralization, and ensuring vaccine safety through regulatory testing, all within GMP-compliant environments. Adept at managing multiple tasks while maintaining high accuracy, attention to detail, and adherence to quality standards.

Available upon request.

• Identification of Species Adulteration in Canned Tuna Products using Triplex PCR Technique, 2018, Developed a robust and reliable authentication tool using a triplex PCR technique to detect species adulteration in commercially available canned tuna products., Conducted DNA extraction from Tuna samples using the DNeasy mericon food kit by QIAGEN (Germany), ensuring accurate and consistent DNA recovery., Used Spectrophotometer and fluorometer analysis to validate the extracted DNA's quality and purity, guaranteeing the accuracy of subsequent PCR analysis., Utilised conventional triplex PCR methodology to target the cytochrome b gene in tuna, enabling precise species identification., Verified the accuracy of species identification by performing 2% Agarose gel electrophoresis, confirming the presence of the Tuna species., Published the findings of this research at the Vingnanam International Research Conference in 2018, hosted by the University of Jaffna.
• Detection of Lactobacillus Genus in Commercially Available Cheese Products and Analysis of Their Antibiotic Sensitivity, 2018, Cultured commercially available cheese samples on MRS agar and employed gram staining and catalase tests for accurate identification of Lactobacillus positive colonies., Utilised manual DNA extraction methods, including the CTAB method and phenol-chloroform method, to extract DNA from identified Lactobacillus colonies., Conducted PCR to analyze antibiotic resistance by amplifying the tet(M) and erm(B) resistance genes, providing insights into antibiotic sensitivity within the Lactobacillus genus.